| 台湾黑僵菌F061菌株孢外酶活性及其对柑橘粉蚧毒力的相关性 |
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| 引用本文： | 黄鹏,姚锦爱,林勇文,余德亿.台湾黑僵菌F061菌株孢外酶活性及其对柑橘粉蚧毒力的相关性[J].福建农业学报,2017,32(1). |
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| 作者姓名： | 黄鹏 姚锦爱 林勇文 余德亿 |
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| 作者单位： | 1. 福建省农业科学院植物保护研究所,福建福州 350013;福建省作物有害生物监测与治理重点实验室,福建福州 350012;2. 福建农林大学植物保护学院,福建 福州,350002 |
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| 基金项目： | 福建省科技计划项目——省属公益类科研院所基本科研专项，国家星火计划项目，福建省区域发展项目，福建省农业科学院科青年人才创新基金项目 |
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| 摘 要： | 室内测定台湾黑僵菌F061菌株的孢外酶活性及其对柑橘粉蚧的毒力,并通过线性回归分析探讨两者的相关性。结果表明:供试菌株的2种胞外酶活性均在1.00×10~8和1.00×107孢子·mL~(-1)2个处理时较高,其中蛋白酶活性达7.63U·mL~(-1)和7.61U·mL~(-1),几丁质酶活性达5.45U·mL~(-1)和5.67U·mL~(-1);供试菌株对柑橘粉蚧的LT50也在1.00×10~8和1.00×107孢子·mL~(-1)2个处理时较短,分别为4.98d和5.39d;线性回归分析表明,供试菌株对柑橘粉蚧的LT50与菌株蛋白酶活性呈显著负相关,与菌株几丁质酶活性呈中度负相关。可见,台湾黑僵菌F061菌株蛋白酶活性比几丁质酶活性强,在侵染柑橘粉蚧过程中起更直接的作用。
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| 关 键 词： | 台湾黑僵菌 孢外酶活性 柑橘粉蚧 毒力 相关性 |
Correlation Between Ectoenzyme Activity and Virulence against Planococcus citri of Metarhizium anisopliae F061 |
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| HUANG Peng,YAO Jinai,LIN Yong-wen,YU De-yi.Correlation Between Ectoenzyme Activity and Virulence against Planococcus citri of Metarhizium anisopliae F061[J].Fujian Journal of Agricultural Sciences,2017,32(1). |
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| Authors: | HUANG Peng YAO Jinai LIN Yong-wen YU De-yi |
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| Abstract: | The ectoenzyme activity and the virulence against Planococcus citri of Metarhizium anisopliae F061 were determined in the laboratory.Their correlation was established using a linear regression analysis.The activities of two ectoenzymes from the bacteria were high.At concentrations of 1.00 × 108 and 1.00)× 107 conidia · mL 1,the protease activities of the spores were 7.63 U · mL-1 and 7.61 U · mL-1,respectively;while the chitinase activities,5.45 U · mL-1 and 5.67 U · mL 1,respectively;and,the LT50 of the strain against P.citri,4.98 d and 5.39 d,respectively.The LT50inversely correlated significantly with the protease activity,but moderately with the chitinase activity.It appeared that the protease were stronger than the chitinase in M.anisopliae F061,and thus,played a more critical role in infectingP.citri. |
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| Keywords: | Metarhizium anisopliae ectoenzyme Planococcus citri virulence correlation |
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