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不同生长阶段细鳞鲑(Brachymystax lenok)消化酶活性比较研究
引用本文:徐革锋,刘洋,谷伟,牟振波.不同生长阶段细鳞鲑(Brachymystax lenok)消化酶活性比较研究[J].东北农业大学学报,2012(12):109-114.
作者姓名:徐革锋  刘洋  谷伟  牟振波
作者单位:中国水产科学研究院黑龙江水产研究所
基金项目:“十二五”国家科技支撑计划(2012BAD25B10,2012BAD26B00);中央级公益性科研院所基本科研业务费专项(201002)
摘    要:对处于不同生长阶段细鳞鲑消化道的蛋白酶、淀粉酶和脂肪酶的活性及其分布进行初步研究。结果表明,在幼鱼、鱼种和亲鱼3个主要生长阶段,细鳞鲑胃内蛋白酶活性最高,幼鱼期蛋白酶活性略低,鱼种和亲鱼期维持在同一水平;淀粉酶活性的变化趋势是幼鱼、鱼种期逐渐增大,亲鱼期达到最大值;脂肪酶活性在幼鱼和鱼种期最高,亲鱼期的脂肪酶活性较低。3种消化酶在细鳞鲑不同器官的分布特点为,蛋白酶活性在胃部最强,其分布趋势为食道<幽门盲囊<肠道<胃;淀粉酶在各个消化区段均有分布,在前肠部位淀粉酶活性最高,在幽门盲囊和后肠处活性最低;胃内脂肪酶活性最高,从胃向后的消化道各主要部位的脂肪酶活性变化呈递减趋势,胃内最高,幽门部次之,肠道最低。

关 键 词:细鳞鲑  肠道  消化酶  比活力

Study on digestive enzyme activities of Brachymystax lenok at differ ent growth stages
XU Gefeng,LIU Yang,GU Wei,MOU Zhenbo.Study on digestive enzyme activities of Brachymystax lenok at differ ent growth stages[J].Journal of Northeast Agricultural University,2012(12):109-114.
Authors:XU Gefeng  LIU Yang  GU Wei  MOU Zhenbo
Institution:(Heilongjiang River Fishery Research Institute,Chinese Academy of Fishery Science,Harbin 150070,China)
Abstract:Distribution and peculiarity of digestive enzyme in the alimentary system of Brachy-mystax lenok were detected in this study.Fish in three developmental stages were used,including juvenile,fingerling and reproductive fish.Protease,amylase and lipase were mainly investigated in this study.The results showed that the activity of protease was the highest among these digestive enzymes in stomach;the activity of protease in juvenile fish was lower than that in fingerling and reproductive fish,which were maintained at the same level.The activity of amylase increasing from juvenile to fingerling stage,until parent fish was the highest.The activity of lipase had high level in juvenile and fingerling fish,then lessened down in the reproductive fish.Distribution of three digestive enzymes activities in the alimentary system of B.lenok was studied.The activity of protease was the highest in the pylorus,and the distribution trend in different organs was esophagus
Keywords:Brachymystax lenok  intestine  digestive enzyme  specific activity
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