In vitro clonal propagation of mature Tectona grandis through axillary bud proliferation |
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| Authors: | Fatima Shirin Prem Kumar Rana Asim Kumar Mandal |
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| Institution: | (1) Genetics and Plant Propagation Division, Tropical Forest Research Institute, PO RFRC, Mandla Road, Jabalpur, 482021, M.P., India;(2) Tropical Forest Research Institute, Jabalpur Madhya Pradesh, India |
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| Abstract: | An in vitro clonal propagation procedure for mature Tectona grandis (teak) trees is described. Multiple shoots were induced from nodal segments through axillary bud proliferation. A shoot multiplication
rate of 6.33 was achieved on Murashige and Skoog's (MS) medium supplemented with 10 μM 6-benzyladenine BA and 1 μM 1-naphthalene
acetic acid (NAA) during every subculture cycle of 4 weeks. In vitro raised shoots could be successfully rooted (66.66%) on
liquid MS medium supplemented with 15 μM NAA, with 1.60 roots per shoot, every 6 weeks of culture. In vitro hardening was
carried out in sand soaked with half-strength MS medium (organic free). The plantlets were acclimatized first in a mist chamber
and then in polybags in a mixture of soil, sand, and farmyard manure (1 : 1 : 1 v/v) in a shade house. |
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| Keywords: | Axillary bud proliferation Clonal propagation In vitro Mature Tectona grandis |
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