| 益生芽孢杆菌B.licheniformis29质粒pBL29抗性基因的筛选 |
| |
| 引用本文： | 刘一尘,倪学勤,王淑芳,何明清,李宏伟.益生芽孢杆菌B.licheniformis29质粒pBL29抗性基因的筛选[J].河南科技大学学报(农学版),2003,23(1):36-39. |
| |
| 作者姓名： | 刘一尘 倪学勤 王淑芳 何明清 李宏伟 |
| |
| 作者单位： | 1. 河南科技大学,动物科技学院,河南,洛阳,471003
2. 四川农业大学,动物科技学院,四川,雅安,625014 |
| |
| 摘 要： | 采用改良碱裂解法对30株益生芽孢杆菌进行质粒抽提，仅从1株地衣芽孢杆菌B．licheniformis29菌株中抽提得到质粒pBL29，纯化后通过琼脂糖凝胶电泳观察，确证质粒pBL29为闭合环状、分子量3．7kb左右。通过药敏试验、吖啶橙消除试验、十二烷基磺酸钠消除试验及原生质体转化试验证明：B．licheniformis29菌株具有卡那霉素抗性。而且卡那霉素抗性基因是质粒编码而不是染色体编码。
|
| 关 键 词： | 益生芽孢杆菌 质粒 pBL29抗性基因 原生质体 基因转移 卡那霉素 改良碱裂解法 质粒抽提 |
| 文章编号： | 1008-4673(2003)01-0036-04 |
| 修稿时间： | 2003年1月5日 |
Resistance gene for plasmid pBL29 of B.licheniformis 29 |
| |
| LIU Yi-chen,NI Xue-qin,WANG Shu-fang,HE Ming-qing,LI Hong-wei.Resistance gene for plasmid pBL29 of B.licheniformis 29[J].Journal of Luoyang Agricultural College,2003,23(1):36-39. |
| |
| Authors: | LIU Yi-chen NI Xue-qin WANG Shu-fang HE Ming-qing LI Hong-wei |
| |
| Abstract: | The presence of plasmid in Bacillus was isolated by improved alkaline lysis. Only one plasmid pBL29 was detected in B. Iicheniformis29. Puried and identified by gel electrophoresis, the plasmid pBL29 is cccDNA and the molecular weight'of the plasmid pBL29 was estimated to be about 3.7kb. The sensitivity of Bacillus Iicheniformis29 to 18 antibiotics was determined by inhibitory zone with filter paper. The result proves that B. Iicheniformis29 is resistant to Kan and sensitive to the other antibiotics. The elimination of plasmid by acridine orange or by SDS and the transformation of protoplast indicated that Kan resistance genes are located on the plasmid pBL29, not on the chromosome. |
| |
| Keywords: | Plasmids pBL29 Kan-resistance Resistance factor Protoplasts Transgenosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
