| 水稻侧根突变体Oslrd2的表型分析和基因克隆 |
| |
| 引用本文： | 朱俊兆,陈宇杰,吕沈阳,陈星月,杨愈之,郑文娟,朱世华,丁沃娜.水稻侧根突变体Oslrd2的表型分析和基因克隆[J].核农学报,2022,36(3):535-543. |
| |
| 作者姓名： | 朱俊兆 陈宇杰 吕沈阳 陈星月 杨愈之 郑文娟 朱世华 丁沃娜 |
| |
| 作者单位： | 1宁波大学科学技术学院, 浙江 宁波 3152122宁波大学海洋学院,浙江 宁波 315211 |
| |
| 基金项目： | 国家自然科学基金(32071981)；;宁波市自然科学基金(202003N4016)； |
| |
| 摘 要： | 侧根是水稻胚后发育的重要器官,具有吸收养分和稳固植株的作用.本研究从甲基磺酸乙酯(EMS)诱变的水稻Kasalath突变体库中筛选得到一个水稻侧根突变体Oslrd2,对其进行了表型和遗传分析、图位克隆、候选基因测序及转基因互补验证.结果表明,与野生型相比,Oslrd2幼苗期的苗高和根系伸长都受到明显抑制,侧根短且数量少...
|
| 关 键 词： | 水稻 侧根突变体 遗传分析 图位克隆 功能互补 |
| 收稿时间： | 2021-01-18 |
Phenotypic Analysis and Gene Cloning of a Lateral Root Mutant Oslrd2 in Rice(Oryza sativa L.) |
| |
| ZHU Junzhao,CHEN Yujie,LYU Shenyang,CHEN Xingyue,YANG Yuzhi,ZHENG Wenjuan,ZHU Shihua,DING Wona.Phenotypic Analysis and Gene Cloning of a Lateral Root Mutant Oslrd2 in Rice(Oryza sativa L.)[J].Acta Agriculturae Nucleatae Sinica,2022,36(3):535-543. |
| |
| Authors: | ZHU Junzhao CHEN Yujie LYU Shenyang CHEN Xingyue YANG Yuzhi ZHENG Wenjuan ZHU Shihua DING Wona |
| |
| Institution: | 1College of Science and Technology, Ningbo University, Ningbo, Zhejiang 3152122School of Marine Science, Ningbo University, Ningbo, Zhejiang 315211 |
| |
| Abstract: | Lateral roots are important organs for rice post-embryonic development,which play roles in nutrients absorptions and plant stabilization.In this study,a rice lateral root mutant Oslrd2(Oryza sativa lateral root defective 2)was identified from a mutant library derived from Kasalath by ethyl methyl sulfonate(EMS).The phenotypes of the mutant were studied and genetic analysis,map-based cloning,candidate gene sequencing and transgenic complementation were conducted.Compared with the wild type,the seedling height and root elongation of Oslrd2 was clearly inhibited in the seedling stage,and the lateral roots were also less and shorter.Through methylene blue staining and anatomical examination,the lateral root primordia in Oslrd2 were less,and the primordia and lateral roots were swelled.Moreover,the plant height,effective panicles number,the number of grains per panicle,and thousand-grain weight of Oslrd2 at the maturation stage were significantly impaired.Genetic analysis showed that the mutant phenotype of Oslrd2 was controlled by a pair of recessive nuclear genes.And the mutant gene was located between two markers InDel 3 and InDel 4 on rice chromosome 11 with a physical distance of about 10 kb.A gene encodingα-tubulin(LOC_Os11g14220)was found within this region.Sequencing analysis revealed a deletion of C1176 and a transition of C1179 to A in its CDS sequence of Oslrd2,which resulted in the replacement of residue D392 by E392 and I393 by a stop codon in the encoded protein sequence,respectively,leading to premature termination of translation.Complementation test confirmed that the Oslrd2 mutant phenotype was caused by this gene.Our results clarify the critical role of OsLRD2 in rice root development and provide a theoretical basis to dissect the molecular mechanism of lateral root development in rice. |
| |
| Keywords: | Oryza sativa L lateral root mutant genetic analysis map-based cloning functional complementation |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
