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基于代表性植株二次选择和多元统计分析的芦笋芽期苗期耐盐性鉴定及综合评价
引用本文:仪泽会,毛丽萍,赵婧,孙黛珍.基于代表性植株二次选择和多元统计分析的芦笋芽期苗期耐盐性鉴定及综合评价[J].核农学报,2022,36(3):589-601.
作者姓名:仪泽会  毛丽萍  赵婧  孙黛珍
作者单位:1山西农业大学农学院,山西 太原 0300312山西农业大学园艺学院,山西 太原 030031
基金项目:山西省农业科学院农业科技创新研究课题(YCX2020YQ07)。
摘    要:土壤盐渍化是制约农业发展的逆境因素之一,筛选耐盐种质可为芦笋耐盐新品种选育和耐盐机理研究奠定基础.本研究以49个芦笋品种为试验材料,采用二次选择法选择代表性植株,设0 mmol·L-1 NaCl(CK)和310 mmol·L-1 NaCl(T)2个水培处理,分别测定发芽率、发芽势等4个芽期指标和株高、根长等8个苗期指标...

关 键 词:芦笋  代表性植株  耐盐性  鉴定方法  多元统计分析
收稿时间:2021-06-23

Identification and Comprehensive Evaluation for Salt Tolerance of Asparagus Officinalis at Germination and Seedling Stages Based on Secondary Selection of Representative Plants and Multivariate Statistical Analysis
YI Zehui,MAO Liping,ZHAO Jing,SUN Daizhen.Identification and Comprehensive Evaluation for Salt Tolerance of Asparagus Officinalis at Germination and Seedling Stages Based on Secondary Selection of Representative Plants and Multivariate Statistical Analysis[J].Acta Agriculturae Nucleatae Sinica,2022,36(3):589-601.
Authors:YI Zehui  MAO Liping  ZHAO Jing  SUN Daizhen
Institution:1College of Agriculture, Shanxi Agricultural University, Taiyuan, Shanxi 0300312College of Horticulture, Shanxi Agricultural University, Taiyuan, Shanxi 030031
Abstract:Soil salinization is one of the stress factors that impeding the development of agriculture.Screening of salt-tolerant germplasm of asparagus can lay a foundation for breeding new salt-tolerant asparagus varieties and studying the salt-tolerant mechanism.Forty-nine asparagus varieties were used as experimental materials in this study and the representative plants of each variety were selected by the secondary selection method.The representative plants were cultured in hydroponics supplement with 0(CK)and 310 mmol·L-1NaCl(T)for salt stress,respectively.Four germination indicators and eight seedling indicators,such as germination rate,germination potential,plant height,root length,and so on were measured.Based on the salt tolerance coefficient,multiple statistical analyses were used to comprehensive evaluate the salt tolerance of 49 asparagus varieties.The results showed that salt stress had extremely significant effects on all indicators(P<0.01).Among them,the salt tolerance coefficient of underground dry weight and root-shoot ratio were greater than 1,while the other indicators are less than 1.Correlation analysis showed that there was extremely significant correlation among different germination indicators(P<0.01),and most seedling indicators significantly(P<0.05)or extremely significant(P<0.01)correlated with each other.The tested varieties were clustered into four salt-tolerant grades including strong salt tolerance,medium salt tolerance,weak salt tolerance and salt sensitivity according to U values at germination stage.The eight individual indicators at seedling stage were converted into three separate comprehensive indicators by principal component analysis,representing 77.805%of the total information.According to D value clustering analysis,12 strong salt-tolerant varieties,20 medium salt-tolerant varieties,14 weak salt-tolerant varieties and 3 salt-sensitive varieties were identified at seedling stage.Among them,only Guanjun and Texlong were identified as strong salt tolerance at both germination and seedling stage.The ranking of salt-tolerance based on U value at germination stage and D value at seedling stage was basically consistent with the comprehensive salt tolerance coefficient(CSC)and weighted salt tolerance coefficient(WSC)at the same stage.Compared with the traditional identification method,the method used in this study was more scientific and reasonable,which could greatly reduce the workload of field identification during the whole growth period,and could be suitable for the salt-tolerant evaluation of lots of asparagus germplasms and has important significance for the improvement and utilization of saline soil.
Keywords:Asparagus officinalis L  representative plants  salt tolerance  identification method  multivariate statistical analysis
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