| 穿心莲四倍体的诱导及其愈伤组织对肉桂酸胁迫的响应 |
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| 引用本文： | 佘晓环,张梓豪,李明.穿心莲四倍体的诱导及其愈伤组织对肉桂酸胁迫的响应[J].北方园艺,2022(2):94-103. |
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| 作者姓名： | 佘晓环 张梓豪 李明 |
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| 作者单位： | 广东药科大学中药学院,广东广州510006,广东药科大学中药学院,广东广州510006;国家中医药管理局岭南药材生产与开发重点研究室,广东广州510006 |
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| 基金项目： | 广东省科技计划资助项目(2014A020208135)；;国家重点研发计划资助项目(2017YFC1700704)；;广东省重点领域研发计划资助项目(2020B020221002)； |
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| 摘 要： | 以不同倍性穿心莲诱导的愈伤组织为试材,采用秋水仙素对穿心莲种子进行四倍体诱导,通过在穿心莲四倍体及二倍体诱导愈伤组织培养基中添加化感自毒物质肉桂酸进行共培养的方法,研究了肉桂酸对不同倍性穿心莲愈伤组织的褐化率、细胞鲜质量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的影响,以期为筛选穿心莲抗化感自毒物质的优良种质资源提供参考依据。结果表明:用0.05%的秋水仙素处理48h,穿心莲种子诱导成活率达89%,经过植株形态、气孔及染色体数目检测,确定了穿心莲四倍体株系PZ-1、PZ-2、PZ-3、PZ-4、PZ-5、PZ-6、PZ-7、PZ-8,其染色体数为4x=96,二倍体为2x=48。在1.00mmol·L-1肉桂酸的愈伤组织诱导培养基中培养7d,穿心莲四倍体愈伤组织的褐化率和MDA含量较二倍体低,而愈伤组织鲜质量、SOD、POD、CAT活性较二倍体高,显示四倍体较二倍体具有对肉桂酸更强的抵御能力,其中四倍体PZ-7表现出较显著的优异性状。
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| 关 键 词： | 穿心莲 多倍体 肉桂酸 抗性 |
Polyploidy Induction of Andrographis paniculata (Burn.F.) Nees and Its Response to Cinnamic Acid |
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| SHE Xiaohuan,ZHANG Zihao,LI Ming.Polyploidy Induction of Andrographis paniculata (Burn.F.) Nees and Its Response to Cinnamic Acid[J].Northern Horticulture,2022(2):94-103. |
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| Authors: | SHE Xiaohuan ZHANG Zihao LI Ming |
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| Institution: | (College of Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006;Key Laboratory of Production and Development of Lingnan Medicinal Materials,State Administration of Traditional Chinese Medicine,Guangzhou,Guangdong 510006) |
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| Abstract: | The callus induced by different ploidy of Andrographis paniculata(Burn.F.)Nees was used as the test material,and colchicine was used to induce tetraploidy of Andrographis paniculata(Burn.F.)Nees seeds by adding allelopathic autotoxic substances to the culture medium for the callus induction of tetraploid and diploid of Andrographis paniculata(Burn.F.)Nees.The effects of cinnamic acid on the browning rate,cell fresh weight,superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)activity and malondialdehyde(MDA)content of callus of different ploidy Andrographis paniculata(Burn.F.)Nees.,in order to provide a preliminary research foundation for screening excellent germplasm resources of Andrographis paniculata(Burn.F.)Nees against allelopathic and autotoxic substances.The results showed that treated with 0.05%colchicine for 48hours,the induced survival rate of Andrographis paniculata(Burn.F.)Nees seeds reached 89%.After the plant morphology,stomata and chromosome number were tested,the tetraploid lines PZ-1,PZ-2,and PZ-3of Andrographis paniculata(Burn.F.)Nees were determined,PZ-4,PZ-5,PZ-6,PZ-7,PZ-8,the number of chromosomes was 4x=96,and the diploid was 2x=48.After culturing in 1.00mmol·L-1 cinnamic acid callus induction medium for 7days,the browning rate and MDA content of Andrographis paniculata(Burn.F.)Nees tetraploid callus were lower than those of diploid,while the fresh quality of callus,SOD,POD,CAT activities were higher than those of diploid,which showed that tetraploid had stronger resistance to cinnamic acid than diploid.Among them,tetraploid PZ-7showed more remarkable excellent characteristics. |
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| Keywords: | Andrographis paniculata(Burn F )Nees polyploid cinnamic acid resistance |
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