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华恢1号衍生系旱恢3T的选育及抗虫表现
引用本文:叶水烽,楼珏,高宁宁,杨锌,邓接楼.华恢1号衍生系旱恢3T的选育及抗虫表现[J].核农学报,2022,36(1):1-6.
作者姓名:叶水烽  楼珏  高宁宁  杨锌  邓接楼
作者单位:1上饶师范学院生命科学学院,江西 上饶 3340012温州科技职业学院/温州市农业科学研究院 浙南作物育种重点实验室,浙江 温州 3250063上海市农业生物基因中心,上海 201106
基金项目:上海市自然科学基金(18ZR1433400);
摘    要:水稻作为世界上主要的粮食作物之一,容易受到螟虫、稻飞虱、稻象甲等主要害虫为害造成减产.为改良节水抗旱稻的抗虫性,以华恢1号(TT51)为cry1Ab/Ac基因供体,与节水抗旱稻恢复系品种旱恢3号杂交,利用分子标记辅助选择技术对目标基因进行筛选,最终获得BC3F4纯合、稳定株系.通过对Cry1Ab/Ac蛋白的定性和定量检...

关 键 词:抗虫  Cry1Ab/Ac蛋白  节水抗旱稻  杂交
收稿时间:2020-11-23

Production and Insect-Resistant Characterization of Hanhui 3T a Derivative Line of Huahui No.1
YE Shuifeng,LOU Jue,GAO Ningning,YANG Xin,DENG Jielou.Production and Insect-Resistant Characterization of Hanhui 3T a Derivative Line of Huahui No.1[J].Acta Agriculturae Nucleatae Sinica,2022,36(1):1-6.
Authors:YE Shuifeng  LOU Jue  GAO Ningning  YANG Xin  DENG Jielou
Institution:1College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 3340012Southern Zhejiang Key Laboratory of Crop Breeding,Wenzhou Vocational College of Science and Technology/Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang 3250063Shanghai Agrobiological Gene Center, Shanghai 201106
Abstract:As one of the major food crops of the world,rice is susceptible to major pests such as stem borers,rice planthoppers,rice weevil,that cause the reduction of yield.In order to improve the insect resistance of water-saving and drought resistant rice,Huahui No.1(TT51)was used as Cry1Ab/Ac gene donor to cross with Hanhui No.3,a water-saving and drought-resistance rice restorer line.The target gene was screened by molecular marker assisted selection(MAS)technology,and BC3F4homozygous and stable lines were obtained.Through qualitative and quantitative detection of Cry1 Ab/Ac protein,combined with field agronomic traits and insect resistance observation,a new insect resistant restorer line Hanhui 3 T was selected.The results showed that compared with the control Hanhui No.3,Hanhui 3 T had similar agronomic traits,but obvious resistance to Cnaphalocrocis medinalis.The hybrid plants of Huhan 5 A/Hanhui 3 T were also highly resistant to Cnaphalocrocis medinalis,while the control hybrid plants of Huhan 5 A/Hanhui No.3 were damaged by Cnaphalocrocis medinalis.The results of this study provide a material foundation for breeding new insect resistance rice varieties with water saving and drought resistance.
Keywords:insect-resistance  Cry1Ab/Ac protein  water-saving and drought-resistance rice  hybrids
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