| NtCPS2对烟草光合特征及光合产物的影响 |
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| 引用本文： | 贺凌霄,韩文龙,李波,江智敏,徐敏,李芳芳,郑聪,徐世晓.NtCPS2对烟草光合特征及光合产物的影响[J].核农学报,2022,36(3):554-566. |
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| 作者姓名： | 贺凌霄 韩文龙 李波 江智敏 徐敏 李芳芳 郑聪 徐世晓 |
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| 作者单位： | 1河南农业大学烟草学院,河南 郑州 4500022浙江中烟工业有限责任公司,浙江 杭州 3100083中国烟草总公司河南省公司,河南 郑州 4500024福建省烟草公司南平市烟草公司浦城分公司,福建 南平 353400 |
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| 基金项目： | 中国烟草总公司河南公司科技重点项目(2021410000240021);湖南烟草总公司长沙公司科技重点项目(21-23A04);广西壮族自治区烟草总公司百色公司科技重点项目(2012145100020165);福建省烟草公司南平市公司科技重点项目(NYK2021-09-03)。 |
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| 摘 要： | 为探讨顺-冷杉醇合成基因NtCPS2对烟草光合作用的影响,以烤烟品系8306、8306B(纯合突变植株T3代)、新豫烟11号(MSK326×8306B)和烤烟品种MSK326、豫烟11号(MSK326×8306)为试验材料,比较其顺-冷杉醇、赤霉素、脱落酸含量及相关合成基因相对表达量,光合参数及光合作用相关基因 相对表...
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| 关 键 词： | NtCPS2 赤霉素 脱落酸 光合特征 光合产物 |
| 收稿时间： | 2021-09-02 |
Effects of NtCPS2 on Photosynthetic Characteristics and Photosynthates of Tobacco |
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| HE Lingxiao,HAN Wenlong,LI Bo,JIANG Zhimin,XU Min,LI Fangfang,ZHENG Cong,XU Shixiao.Effects of NtCPS2 on Photosynthetic Characteristics and Photosynthates of Tobacco[J].Acta Agriculturae Nucleatae Sinica,2022,36(3):554-566. |
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| Authors: | HE Lingxiao HAN Wenlong LI Bo JIANG Zhimin XU Min LI Fangfang ZHENG Cong XU Shixiao |
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| Institution: | 1College of Tobacco, Henan Agricultural University, Zhengzhou, Henan 4500022China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou, Zhejiang 3100083Henan Company of China National Tobacco Corporation, Zhengzhou, Henan 4500024Pucheng Branch of Nanping Tobacco Company of Fujian Province, Nanping, Fujian 353400 |
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| Abstract: | To investigate the effects of cis-abienol biosynthesis gene NtCPS2 on tobacco photosynthesis,we compared the differences of photosynthetic parameters,enzyme activity,products,stomatal opening,cis-abienol,GA3,ABA contents and related synthetic genes among 8306,8306 B,New Yuyan11(MSK326×8306 B),MSK326 and Yuyan11 MSK326×8306.The results showed that when compared between 8306 B and 8306,Yuyan11 and New Yuan11,the cis-abienol content of 8306 B and Yuyan 11 were lower,the GA3 content was increased by 7.90%and 9.79%,the ABA content was decreased by 35.87%and 11.60%,respectively.Furthermore,the expression level of GA20ox,a key positive regulation gene in GAs synthesis,was significantly increased,whereas the expression levels of negative regulation key gene GA2ox and ABA synthesis genes PSY and NCED were significantly decreased.In other aspects,the stomatal closure ratio was decreased by 43.75%and 8.33%,respectively.The stomatal conductance net photosynthetic rate the activities of photosynthesis-related enzymes Rubisco and GAPDH,and the relative gene expression were increased.The photosynthates were changed.Meanwhile the height,number of leaves and pitch of tobacco plant were increased significantly.These findings indicate that the NtCPS2 gene not only affected the diterpenoid metabolic pathway,reduced the content of cis-abienol and increased the content of GA3,but also affected the photosynthesis improved the photosynthetic efficiency and the plant-type.This research provides an example for the feedback regulation of terpenoids on plant photosynthesis. |
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| Keywords: | NtCPS2 GAs ABA photosynthetic characteristics photosynthetic product |
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