| 脱氧雪腐镰刀菌烯醇及其乙酰化衍生物对Raw264.7细胞的联合毒性效应研究 |
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| 引用本文： | 董燕婕,范丽霞,苑学霞,邵阳阳,王磊,赵善仓.脱氧雪腐镰刀菌烯醇及其乙酰化衍生物对Raw264.7细胞的联合毒性效应研究[J].核农学报,2022,36(1):136-142. |
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| 作者姓名： | 董燕婕 范丽霞 苑学霞 邵阳阳 王磊 赵善仓 |
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| 作者单位： | 山东省农业科学院农业质量标准与检测技术研究所/山东省食品质量与安全检测技术重点实验室, 山东 济南 250100 |
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| 基金项目： | 山东省农业科学院农业科技创新工程产业重大技术创新项目(CXGC2016B17);山东省重大科技创新工程(2019JZZY020903)。 |
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| 摘 要： | 为了解脱氧雪腐镰刀菌烯醇及其乙酰化衍生物混合污染的联合毒性效应,本试验以小鼠腹腔巨噬细胞Raw264.7为体外模型,研究脱氧雪腐镰刀菌烯醇(DON)及其乙酰化衍生物3a-DON和15a-DON对小鼠腹腔巨噬细胞Raw264.7的细胞毒性及其联合毒性效应;体外培养Raw264.7细胞,研究不同浓度梯度(0.090、0.1...
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| 关 键 词： | 细胞毒理学 脱氧雪腐镰刀菌烯醇 乙酰化衍生物 联合毒性 |
| 收稿时间： | 2020-12-07 |
Combined Cytotoxic Effects of Deoxynivalenol and Its Acetyl Ated Derivatives on Raw264.7 Cells |
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| DONG Yanjie,FAN Lixia,YUAN Xuexia,SHAO Yangyang,WANG Lei,ZHAO Shancang.Combined Cytotoxic Effects of Deoxynivalenol and Its Acetyl Ated Derivatives on Raw264.7 Cells[J].Acta Agriculturae Nucleatae Sinica,2022,36(1):136-142. |
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| Authors: | DONG Yanjie FAN Lixia YUAN Xuexia SHAO Yangyang WANG Lei ZHAO Shancang |
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| Institution: | Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan, Shandong 250100 |
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| Abstract: | To investigate the cytotoxicity of deoxynivalenol(DON)and its acetyl ated derivatives,the individual and combined cytotoxicity of deoxynivalenol(DON)and the derivatives(3a-DON and 15a-DON)on Raw264.7 cells were studied in vitro.Raw264.7 cells was treated with different concentration of DON、3a-DON and 15a-DON with different concentration gradients(0.090,0.180,0.375,0.750,1.500,3.000μg·mL-1)acting alone and in binary or ternary combination.The changes of cell activity,reactive oxygen species and mitochondrial membrane potential were measured.The IC50 values of DON、3a-DON and 15 a-DON on Raw264.7 cells were 0.2754、1.8060、0.2671μg·mL-1,respectively.The concentration of reactive oxygen species and the permeability of mitochondrial membrane were increased after the individual and combined action of toxin.The binary and ternary combined toxic effects of DON、3a-DON and 15 a-DON t on Raw264.7 cells were synergistic.As a result,the synergistic effect should be considered in evaluating the risk of DON and its acetylated derivatives. |
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| Keywords: | cytotoxicity deoxynivalenol acetylated derivatives combined toxicity |
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