| 木薯枯草杆菌蛋白酶家族鉴定及MeSDD1的功能分析 |
| |
| 引用本文： | 肖亮,鲍茹雪,曹升,陆柳英,尚小红,曾文丹,聂宣红,严华兵.木薯枯草杆菌蛋白酶家族鉴定及MeSDD1的功能分析[J].核农学报,2022,36(7):1308-1317. |
| |
| 作者姓名： | 肖亮 鲍茹雪 曹升 陆柳英 尚小红 曾文丹 聂宣红 严华兵 |
| |
| 作者单位： | 1广西壮族自治区农业科学院经济作物研究所,广西 南宁 5300072亚热带农业生物资源保护与利用国家重点实验室,广西 南宁 5300073中国热带农业科学院热带生物技术研究所,海南 海口 5711014越南农业科学院, 河内 12500 越南 |
| |
| 基金项目： | 国家重点研发计划项目(2019YFD1001100);国家自然科学基金(31960440);广西自然科学基金重点项目(2020GXNSFDA297031);广西自然科学基金面上项目(2020GXNSFAA259085) |
| |
| 摘 要： | 枯草杆菌蛋白酶基因家族(SBT)是控制植物生长发育和响应环境胁迫的重要基因家族。为进一步了解木薯SBT基因家族的数量、基本特征、功能域和进化关系等,在全基因组水平通过生物信息学方法对SBT基因家族进行了鉴定和分析,并对其中一个成员的功能进行了分析。进化树分析结果表明,在木薯基因组中共鉴定到69个SBT成员,其中Manes.18G044300与拟南芥抗旱基因SDD1亲缘关系最近,因此将该成员命名为MeSDD1;22个成员无内含子,69个成员的蛋白携带5个高度保守的结构域,一部分SBT成员在染色体上呈紧密的串联分布,启动子存在干旱响应元件。用qRT-PCR检测筛选出表达量高的2个纯合株系用于后续研究。相关生理指标检测结果表明,2个转基因株系叶片的相对含水量均显著高于野生型;转基因株系离体叶片失水率小于野生型;转基因株系的气孔密度显著小于野生型;断水处理14 d,转基因株系的萎焉程度轻于野生型,表明MeSDD1增强了拟南芥植株的抗旱性。本研究结果为木薯抗旱育种提供了基因资源,并为后续研究MeSDD1介导的抗旱分子机制奠定了理论基础。
|
| 关 键 词： | 木薯 枯草杆菌蛋白酶家族 转基因拟南芥 抗旱性 功能分析 |
| 收稿时间： | 2021-07-27 |
Identification of Cassava SBT Gene Family and Function Analysis of MeSDD1 |
| |
| XIAO Liang,BAO Ruxue,CAO Sheng,LU Liuying,SHANG Xiaohong,ZENG Wendan,NIE Xuanhong,YAN Huabing.Identification of Cassava SBT Gene Family and Function Analysis of MeSDD1[J].Acta Agriculturae Nucleatae Sinica,2022,36(7):1308-1317. |
| |
| Authors: | XIAO Liang BAO Ruxue CAO Sheng LU Liuying SHANG Xiaohong ZENG Wendan NIE Xuanhong YAN Huabing |
| |
| Institution: | 1Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 5300072State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, Guangxi 5300073Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropic Agricultural Sciences, Haikou, Hainan 5711014Vietnamese Academy of Agricultural Sciences, Hanoi 12500, Vietnam |
| |
| Abstract: | The subtilase (SBT) gene family is an important gene family that controls plant growth and development and responds to environmental stress. In order to understand furtherly the number, basic characteristics, evolutionary relationship of SBT gene family in cassava, the SBT gene family was identified by bioinformatics, and the function of one member of the SBT gene family was characterized. The results showed that a total of 69 SBT members were identified in cassava genome, of which Manes.18G044300 was named MeSDD1 because it is most closely related to Arabidopsis. thaliana SDD1. Of all the SBT members, twenty-two SBT members had no introns, and 69 SBT members carried five highly conserved domains. Some SBT members were tandemly located on chromosomes, and drought response elements were found in their promoters. Two homozygous lines with high expression were screened out by fluorescence quantitative PCR for the subsequent experiments. The results indicated that the relative water contents in leaves of the two transgenic line were significantly higher than that of the wild type (WT), detached leaves of the transgenic lines lost water more solwly than that of WT, and the stomatal density of transgenic lines was significantly lower than that of WT. After water-deficit treatment for 14 d, the wilting degree of transgenic lines was less than that of WT. All the results indicated that MeSDD1 could enhance the drought resistance of A. thaliana. This study will provide gene resources for drought resistance breeding of cassava, and lay a theoretical foundation for the subsequently studying on the molecular mechanism of MeSDD1-mediated drought resistance. |
| |
| Keywords: | cassava subtilase gene family transgenic Arabidopsis thaliana drought resistance functional analysis |
|
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
