| 虫害诱导烟草抗性对斜纹夜蛾取食及解毒酶的影响 |
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| 引用本文： | 余源婵,杨茂发,商胜华,刘健锋,于晓飞,狄雪塬.虫害诱导烟草抗性对斜纹夜蛾取食及解毒酶的影响[J].核农学报,2022,36(1):236-243. |
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| 作者姓名： | 余源婵 杨茂发 商胜华 刘健锋 于晓飞 狄雪塬 |
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| 作者单位： | 1贵州山地农业病虫害重点实验室/贵州大学昆虫研究所,贵州 贵阳 5500252贵州大学烟草学院,贵州 贵阳 5500253贵州省烟草科学研究院,贵州 贵阳 550081 |
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| 基金项目： | 国家自然科学基金项目(31960540)； |
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| 摘 要： | 为探究不同虫害诱导抗性烟草(Nicotiana tabacum L.)对斜纹夜蛾(Spodoptera litura Fabricius)取食和解毒生理的影响,本研究以烤烟品种雄性不育K326为供试材料,观察斜纹夜蛾幼虫对棉铃虫Helicoverpa armigera(Hübner)]诱导烟草、斜纹夜蛾诱导烟草、烟蚜...
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| 关 键 词： | 烟草 诱导抗性 斜纹夜蛾 取食选择 解毒酶 |
| 收稿时间： | 2021-03-01 |
Effects of Herbivore Induced Tobacco Resistance on Feeding and Detoxification Enzymes of Spodoptera litura (Lepidoptera: Noctuidae) |
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| YU Yuanchan,YANG Maofa,SHANG Shenghua,LIU Jianfeng,YU Xiaofei,DI Xueyuan.Effects of Herbivore Induced Tobacco Resistance on Feeding and Detoxification Enzymes of Spodoptera litura (Lepidoptera: Noctuidae)[J].Acta Agriculturae Nucleatae Sinica,2022,36(1):236-243. |
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| Authors: | YU Yuanchan YANG Maofa SHANG Shenghua LIU Jianfeng YU Xiaofei DI Xueyuan |
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| Institution: | 1Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region/Institute of Entomology, Guizhou University, Guiyang, Guizhou 5500252College of Tobacco Science, Guizhou University, Guiyang, Guizhou 5500253Guizhou Academy of Tobacco Science, Guiyang, Guizhou 550081 |
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| Abstract: | In order to investigate the effects of tobacco with different pest-induced resistance on feeding and detoxification physiology of Spodoptera litura, tobacco male sterility K326 was used in this study to observe the feeding selection of S. litura larvae on tobacco induced by Helicoverpa armigera, S. litura and Myzus persica, mechanical treatments(pricking, needling) and untreated tobacco as positive and negative controls. The detoxifying enzymes activities of the following feeder S. litura containing acetylcholinesterase(AchE), carboxylesterase(CarE), glutathione-S-transferase(GST), multifunctional oxidase(MFO) were detected and analyzed. The results showed that the newly hatched larvae of S. litura preferred to eat the positive contrast leaves induced by mechanical pricks and aphids, while the 3 rd instar larvae preferred the positive contrast(punch) leaves relative the S. litura. The AchE activity of S. litura was induced after feeding tobacco leaves, the AchE in aphid processing was exhibited the maximum increase, which was 65.17% higher than that in CK at 48 h. Compared with CK, the H. armigera, S. litura and aphid treatments induced CarE, MFO, and GST at 48, 48 and 4 h increased 14.38%, 72.22% and 47.47% respectively. The activities of MFO and GST, CarE, MFO increased at 12 h and 48 h, 48 h, 4 h, respectively, and there was no significant difference compared with CK(P> 0.05). GST activity of S. litura and CarE activity of M. persicae were significantly inhibited at 48 h, only 55.67% and 81.05% of CK. This indicated that different resistant tobaccos of S. litura have different feeding rates. Feeding resistant tobaccos could activate the AchE of S. litura. Other enzyme under different resistance treatments showed specific changes over time. This study can provide ideas and research basis for integrated control of S. litura in tobacco fields. |
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| Keywords: | tobacco induced resistance Spodoptera litura feeding choice detoxifying enzymes |
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