| 蜡样芽孢杆菌芽孢萌发及其对常规灭菌方法抵抗力的研究简 |
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| 引用本文： | 陈未,周伟伟,陈飞,徐蓓蓓,王丽芳,吴福平,崔金鑫,袁维峰.蜡样芽孢杆菌芽孢萌发及其对常规灭菌方法抵抗力的研究简[J].中国畜牧兽医,2014,41(1):192-195. |
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| 作者姓名： | 陈未 周伟伟 陈飞 徐蓓蓓 王丽芳 吴福平 崔金鑫 袁维峰 |
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| 作者单位： | （1.江苏农牧科技职业学院，江苏泰州 225300；2.张家港出入境检验检疫局，江苏张家港 215600；3.中国农业科学院北京畜牧兽医研究所，北京 100193） |
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| 基金项目： | 江苏检验检疫科研项目（2011KJ05）；国家高技术研究发展计划（863计划）（2012AA101302）；公益性行业（农业）科研专项（200903027、201303042）；中央级公益性科研院所基本科研业务费专项资金（2011js-3）。 |
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| 摘 要： | 芽孢杆菌属细菌在芽孢状态下，对外界环境及理化因素具有高度抵抗力。适宜的温度、湿度及营养条件能促进芽孢萌发，影响其对常规灭菌方法的抵抗力。本试验选取蜡样芽孢杆菌作为研究对象，在适宜培养条件下使其形成芽孢，通过研究萌发后芽孢对温度、H2O2及含氯消毒剂等条件的抵抗力，证实了适当的条件（温度37 ℃、湿度40%、pH 7.4、30 min）能有效促进蜡样芽孢杆菌芽孢萌发，致使芽孢对高温（80 ℃、2 h）、H2O2（1.5 mol/L、2 h）及含氯消毒剂（有效氯含量约800 mg/L、2 h）等常规灭菌方法抵抗力明显降低，并能被有效杀灭和灭活，相对有效杀灭率分别达98.4%、98.9%及99.1%，有效杀灭率分别为88.3%、88.7%及88.9%，这对进一步研究芽孢杆菌属细菌的杀菌消毒条件具有重要参考意义。
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| 关 键 词： | 蜡样芽孢杆菌 芽胞 抵抗力 萌发 常规灭菌方法 |
Research on Bacillus cereus Spore Germination and the Resistance to Conventional Sterilization Methods |
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| CHEN Wei,HOU Wei-wei,CHEN Fei,XU Bei-bei,WANG Li-fang,WU Fu-ping,CUI Jin-xin,YUAN Wei-feng.Research on Bacillus cereus Spore Germination and the Resistance to Conventional Sterilization Methods[J].China Animal Husbandry & Veterinary Medicine,2014,41(1):192-195. |
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| Authors: | CHEN Wei HOU Wei-wei CHEN Fei XU Bei-bei WANG Li-fang WU Fu-ping CUI Jin-xin YUAN Wei-feng |
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| Institution: | （1.Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China；2.Zhangjiagang Inspection and Quarantine Bureau,Zhangjiagang 215600,China；3.Insititute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China） |
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| Abstract: | The Bacillus cereus spore had a strong resistance against the environment and physical and chemical factors. Appropriate conditions of temperature, moisture and nutrition could promote the spore germination and affect resistance of spore to conventional sterilization method. This research selected Bacillus cereus as the research object, made it spore form under the appropriate condition, and study the resistance of germinated spore under the conditions of high temperature、H2O2 and chlorine disinfectants. The results confirmed that appropriate conditions (37 ℃,humidity 40%, pH 7.4, 30 min) could effectively promote the Bacillus cereus spore germination. And the resistance of germinated spore decreased apparently under the conditions such as high temperature (80 ℃,2 h), H2O2 (1.5 mol/L,2 h) or chlorine disinfectants (available chlorine content was 800 mg/L,2 h). The relatively effective killing rates were 98.4%, 98.9% and 99.1%, respectively. And the effective kill rates were 88.3%, 88.7% and 88.9%, respectively. The results had important reference meanings to further research on sterilization conditions of Bacillus genus bacillus. |
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| Keywords: | Bacillus cereus spore resistance germination conventional sterilization method |
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