| 鲍曼不动杆菌雏鸡分离株CCGGD201101的分离、鉴定及致病性研究简 |
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| 引用本文： | 邹德颖,刘东,郭兴,陈晓峰,胡盼,卢士英,周玉,柳增善,任洪林,于师宇.鲍曼不动杆菌雏鸡分离株CCGGD201101的分离、鉴定及致病性研究简[J].中国畜牧兽医,2014,41(1):178-181. |
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| 作者姓名： | 邹德颖 刘东 郭兴 陈晓峰 胡盼 卢士英 周玉 柳增善 任洪林 于师宇 |
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| 作者单位： | （1.人兽共患病研究教育部重点实验室，吉林大学人兽共患病研究所，吉林大学动物医学学院，吉林长春 130062；2.福建出入境检验检疫局，福建福州 350001） |
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| 摘 要： | 试验旨在鉴定吉林省某雏鸡孵育基地病死雏鸡组织中分离出的1株致病性菌CCGGD201101株并测定其致病性。对疑似致病菌进行生理生化试验、16S rDNA测序鉴定，并人工接种昆明鼠，测定其半数致死量，验证细菌毒力。经鉴定该菌为鲍曼不动杆菌（Acinetobacter baumannii）。以鲍曼不动杆菌CCGGD201101分离株为研究对象，并以鲍曼不动杆菌标准株（ATCC 19606）为对照，测得半数致死量，进一步证明鲍曼不动杆菌病死鸡分离株CCGGD201101具有较强致病性。
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| 关 键 词： | 鲍曼不动杆菌 生理生化鉴定 16S rDNA 半数致死量 |
Isolation,Identification and Pathogenicity of Acinetobacter baumannii Strain CCGGD201101 Isolated from Chicks (Gallus gallus domesticus) |
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| ZOU De-ying,LIU Dong,GUO Xing,CHEN Xiao-feng,HU Pan,LU Shi-ying,ZHOU Yu,LIU Zeng-shan,REN Hong-lin,YU Shi-yu.Isolation,Identification and Pathogenicity of Acinetobacter baumannii Strain CCGGD201101 Isolated from Chicks (Gallus gallus domesticus)[J].China Animal Husbandry & Veterinary Medicine,2014,41(1):178-181. |
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| Authors: | ZOU De-ying LIU Dong GUO Xing CHEN Xiao-feng HU Pan LU Shi-ying ZHOU Yu LIU Zeng-shan REN Hong-lin YU Shi-yu |
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| Institution: | （1.Key Laboratory of Zoonosis Research, Ministry of Education， Institute of Zoonosis， College of Veterinary Medicine, Jilin University，Changchun 130062, China;2.Fujian Entry-Exit Inspection & Quarantine Bureau of P.R.C, Fuzhou 350001, China） |
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| Abstract: | The study was aimed to identify the virulent strain CCGGD201101 isolated from dead chicks of a chick farm in Jilin province and determine its pathogenicity. The suspected pathogens were identified by means of physiological and biochemical tests and 16S rDNA sequencing. The median lethal dose to Kunming mice was determined to verify bacterial virulence.The results showed that this strain was Acinetobacter baumannii. The half lethal dose was determined with Acinetobacter baumannii CCGGD201101 as target while Acinetobacter baumannii standard strain (ATCC 19606) as control. The pathogenicity of Acinetobacter baumannii strain CCGGD201101 was further confirmed. |
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| Keywords: | Acinetobacter baumannii physiological and biochemical identification 16S rDNA LD50 |
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