| 鹿白细胞抗菌肽的体外抗菌试验 |
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| 引用本文： | 陈丽颖,杨国宇,王艳玲,杨霞,金钺.鹿白细胞抗菌肽的体外抗菌试验[J].中国兽药杂志,2007,41(7):13-15,18. |
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| 作者姓名： | 陈丽颖 杨国宇 王艳玲 杨霞 金钺 |
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| 作者单位： | 河南农业大学牧医工程学院,河南郑州,450002 |
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| 摘 要： | 应用氯化铵裂解、超声波破碎和离子交换层析等方法，从鹿白细胞中提取出阳离子抗菌肽混合物。经平板抑菌试验初步检测其抗菌活性后，采用微量反应板法测定了鹿抗菌肽对大肠杆菌、金黄色葡萄球菌、白色念珠菌等9个标准菌株的最小抑菌浓度（MIC），结果显示，该混合抗菌肽对革兰氏阳性细菌、革兰氏阴性细菌和真菌均具有抑制活性。体外杀菌曲线试验表明，鹿抗菌肽的杀菌作用是非浓度依赖性的，杀菌效果迅速，可在2min内杀灭63．8％的受试细菌。
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| 关 键 词： | 鹿白细胞 抗菌肽 杀菌曲线 |
| 文章编号： | 1002-1280（2007）07-0013-04 |
| 修稿时间： | 2007-01-22 |
In vitro Bactericidal Experiments of Antimicrobial Peptides from Deer Leukocytes |
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| CHEN Li-ying,YANG Guo-yu,WANG Yan-ling,YANG Xia,JIN Yue.In vitro Bactericidal Experiments of Antimicrobial Peptides from Deer Leukocytes[J].Chinese Journal of Veterinary Drug,2007,41(7):13-15,18. |
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| Authors: | CHEN Li-ying YANG Guo-yu WANG Yan-ling YANG Xia JIN Yue |
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| Institution: | College of Animal Husbandry and Veterinary Sciences, Henan Agricultural University, Zhengzhou, Henan 450002 ; China |
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| Abstract: | Cationic antimicrobial peptides were extracted from deer neutrophils through NH4Cl lyses,sonication and ion-exchange chromatography.Minimal Inhibition Concentrations(MICs) of the extract were determined against 9 standard strains such as E.coli,S.aureus and C.albicans by means of microdilution.The results showed that the extract of peptides was active for Gram-positive,Gram-negative bacteria and yeast.The in vitro time-kill curve experiments revealed that the killing potential of the peptides was concentration-independent and immediate,with 63.8% of the test bacteria killed in 2 minutes. |
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| Keywords: | MIC |
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