| 菊花品种神马的茎段离体快繁研究 |
| |
| 引用本文： | 张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014(12):19-22. |
| |
| 作者姓名： | 张天静 雷家军 孙文松 |
| |
| 作者单位： | 1. 辽宁省经济作物研究所,辽宁辽阳,111000
2. 沈阳农业大学园艺学院,辽宁沈阳,110866 |
| |
| 基金项目： | 沈阳市农业科技共建资助项目(201423) |
| |
| 摘 要： | 为加快优质菊花种苗的繁殖,以菊花品种神马的茎段为试材,建立了无菌操作体系,并进行继代增殖、生根培养和驯化移栽。结果表明:升汞消毒茎段5min效果最佳,存活率可达70.0%,污染率为13.30%;最佳初代培养基为MS+BA 1.0mg·L-1+NAA 0.1mg·L-1,萌发率高且生长良好;最佳增殖培养基为MS+BA1.0mg·L-1+NAA0.1mg·L-1,增殖系数达到6.2;最佳生根培养基为1/2MS+NAA0.1mg·L-1,生根率可达97.8%。
|
| 关 键 词： | 菊花 茎段 离体快繁 |
Study on Stem Micropropogation of Chrysanthemum cv.Jinba |
| |
| ZHANG Tian-jing
,
LEI Jia-jun
,
SUN Wen-song.Study on Stem Micropropogation of Chrysanthemum cv.Jinba[J].Heilongjiang Agricultural Science,2014(12):19-22. |
| |
| Authors: | ZHANG Tian-jing LEI Jia-jun SUN Wen-song |
| |
| Institution: | ZHANG Tian-jing , LEI Jia-jun ,SUN Wen-song (1. Economic Crop Institution of Liaoning Province, Liaoyang, Liaoning 111000;2. College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866) |
| |
| Abstract: | In order to speed up the breeding of high-quality Chrysanthemumcv.Jinba,taking stem segment of Chrysanthemumcultivar Jinba materials,sterile system was established,and the axillary bud in vitro was subcultured,rooted,domesticated and transplanted.The results showed that the disinfection time by 0.1% HgCl2 for 5minutes was optimal,and the survival rate could be up to 70.0%,and contamination rate was 13.3%.When the induction medium was MS＋BA 1.0mg·L-1＋NAA 0.1mg·L-1,the germination rate was high and the plantlets grew well.The optimal subculture medium was MS＋BA1.0mg·L-1＋NAA0.1mg·L-1 with multiplication coefficient of 6.2,and the most suitable rooting medium was 1/2MS＋NAA0.1mg·L-1,with rooting rate over 97.8%. |
| |
| Keywords: | Chrysanthemumcv Jinba stem rapid propogation in vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
