| 麝鼠香囊腺形态学观察及腺上皮体外培养初报 |
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| 引用本文： | 姜 峰,张学明,韩小虎,韩春田,岳占碰,邓旭明.麝鼠香囊腺形态学观察及腺上皮体外培养初报[J].中国农学通报,2006,22(11):40-40. |
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| 作者姓名： | 姜 峰 张学明 韩小虎 韩春田 岳占碰 邓旭明 |
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| 作者单位： | 吉林大学畜牧兽医学院,长春,130062 |
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| 基金项目： | 吉林大学博士科研启动基金项目“麝鼠香囊腺干细胞的分离培养及其产麝香成份可行性研究”（2006006）. |
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| 摘 要： | 取泌香期成年麝鼠香囊腺或对非泌香期麝鼠进行人工诱导香腺发育后取材，在形态学观察的基础上，分离香囊腺上皮细胞并进行体外初步培养。结果显示，用1mg/ml Ⅳ型胶原酶和0.25mg/ml胰蛋白酶分次于37℃下消化组织块5h和15min，可得到大量的分散单细胞用于原代培养；用含10%胎牛血清和双抗的DME/F12培养液为基础液，在培养液中添加10ng/ml表皮生长因子(EGF)，对麝鼠香囊腺上皮细胞的存活和生长具有较明显的促进作用；腺上皮细胞可进行传代培养，也可用常规方法进行冷冻保存和复苏；体外传代培养到第5代的上皮细胞仍增殖旺盛，细胞内有丰富的脂滴，细胞分泌活动旺盛。提示，通过体外分离培养麝鼠香囊腺上皮细胞，建立香囊腺干(前体)细胞系，进而用细胞工程方法大量生产人工麝鼠香是可能的。
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| 关 键 词： | 大豆 大豆 功能性食品 贮藏 加工品质 |
| 收稿时间： | 2006-07-18 |
| 修稿时间： | 2006-07-182006-08-07 |
Morphology of Muskrat Scent Glands and Initial Culture of the Glands Epithelium |
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| Jiang Feng,Zhang Xueming,Han Xiaohu,Han Chuntian,Yue Zhanpeng,Deng Xuming.Morphology of Muskrat Scent Glands and Initial Culture of the Glands Epithelium[J].Chinese Agricultural Science Bulletin,2006,22(11):40-40. |
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| Authors: | Jiang Feng Zhang Xueming Han Xiaohu Han Chuntian Yue Zhanpeng Deng Xuming |
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| Institution: | College of Animal Science and Veterinary Medicine, Jilin University, Chang ehun 130062 |
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| Abstract: | The cystglands of matured muskrat in secreting or the cystglands induced secreting artificially were used. Based on the morphological observation, epithelial cells of the scent glands were isolated and cultured in vitro initially. The results showed that a great quantity of dispersed cells in single state was available for primary culture by digestion with 1mg/ml Collagenase IV for 5 h and 0.25mg/ml Trypsin for 15min at 37℃; DME/F12 containing 10% fetal bovine serum, penicillin(100U/ml)and streptomycin(100U/ml), as well as EGF at the concentration of 10ng/ml, could obviously improve the survival and proliferation of these cells; the epithelial cells could be serial subcultivated, freezed and thawed routinely as well; after the 5th passage, the epithelial cells were filled with many lipid dropleds, secreting and proliferating perfectly. These results suggest that it is feasible to produce artificial muskrat scent using cell engineering by isolating, culturing cystglanitd epithelial cell and furthmore establishing a musdrat cystgland epithelial cell line. |
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| Keywords: | Cystgland Epithelial cell of cystgland Cultured in vitro Muskrat |
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