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无氮饲粮中铬添加水平对大鼠器官指数、肠道黏膜形态及血清指标的影响
引用本文:郑灿财,黄艳玲.无氮饲粮中铬添加水平对大鼠器官指数、肠道黏膜形态及血清指标的影响[J].动物营养学报,2016(1):157-162.
作者姓名:郑灿财  黄艳玲
作者单位:西南民族大学生命科学与技术学院,成都,610041
基金项目:四川省国际合作计划项目,西南民族大学研究生创新型科研项目
摘    要:本试验旨在研究无氮饲粮中添加不同水平丙酸铬(Cr Pro)对Sprague Dawley(SD)大鼠器官指数、肠道黏膜形态及血清指标的影响。试验选用36只体重均一的断奶雌性SD大鼠,分为3个处理,每个处理6个重复,每个重复2只鼠。各处理分别饲喂无氮基础饲粮(对照组,含铬0.08 mg/kg)及在基础饲粮上分别添加0.2和1.0 mg/kg Cr(以Cr Pro形式)的试验饲粮,试验期21 d。结果显示:1)饲粮添加0.2 mg/kg的Cr显著提高了大鼠脾脏指数(P0.05)。2)饲粮添加1.0 mg/kg的Cr显著提高了大鼠空肠绒毛高度与隐窝深度的比值(P0.05)。3)与对照组相比,饲粮添加1.0 mg/kg的Cr显著提高了血清白蛋白和尿酸含量(P0.05),饲粮添加0.2 mg/kg Cr显著提高了血清尿酸含量(P0.05)。由此可见,无氮饲粮中补充适量铬对改善大鼠机体免疫器官指数、肠黏膜形态及血清蛋白质代谢有一定的作用。

关 键 词:无氮饲粮  丙酸铬  器官指数  肠道黏膜  血清指标

Effects of Chromium Supplemented Level in Nitrogen-Free Diets on Organ Indexes,Intestinal Mucosa Morphology and Serum Parameters of Rats
Abstract:This experiment was conducted to investigate the effects of different chromium propionate ( CrPro) level in nitrogen-free diets ( NFD) on organ indexes, intestinal mucosa morphology and serum parameters of rats.According to body weight, a total of 36 weaned Sprague Dawley ( SD) rats were allotted to 3 treatments with 6 replicates per treatment and 2 rats per replicate.Rats were fed a basal NFD ( control group) containing 0.08 mg/kg chromium ( Cr) , meanwhile, the basal diets supplemented with 0.2 or 1.0 mg/kg Cr from Cr for 21 days.The results showed that: 1) dietary supplemented with 0.2 mg/kg Cr significantly improved spleen index ( P<0.05) .2) Dietary supplemented with 1.0 mg/kg Cr significantly increased the ratio of villous height to crypt depth ( P<0.05) .3) Compared with the control group, dietary supplemented with 1.0 mg/kg Cr sig-nificantly improved serum albumin and uric acid contents ( P<0.05) , dietary supplemented with 0.2 mg/kg Cr significantly improved serum uric acid content ( P<0.05) .It is concluded that under NFD condition, the proper Cr supplementation has some effects on improving immune organs indexes, intestinal mucosa morphology and serum protein metabolism.
Keywords:nitrogen-free diets  CrPro  organ indexes  intestinal mucosa  serum parameters
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