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铝胁迫下不同耐铝性小麦根际pH值变化及其与耐铝性的关系
引用本文:杨野,郭再华,叶志娟,耿明建,王伟,宁大伟,赵竹青.铝胁迫下不同耐铝性小麦根际pH值变化及其与耐铝性的关系[J].农业环境科学学报,2010,29(4).
作者姓名:杨野  郭再华  叶志娟  耿明建  王伟  宁大伟  赵竹青
作者单位:1. 华中农业大学资源与环境学院,武汉,430070
2. 武汉软件工程职业学院,武汉,430205
基金项目:国家自然科学基金项目(30571115)
摘    要:Al3+是植物铝毒害的主要形态,而其活性受环境pH值的影响,H+-ATPase通过调节根的质子分泌改变根际pH值。为探讨铝胁迫下根际pH值变化与小麦耐铝性的关系,以小麦品种ET8(耐铝型)、ES8(铝敏感型)为试验材料,采用溶液培养的方法对铝胁迫下根际pH值及根尖H+-ATPase活性变化进行了研究。结果表明,铝处理条件下,小麦根际pH值随培养时间的延长而升高;随培养液中铝浓度的增加,根际pH值上升幅度下降,相同铝浓度处理条件下ET8根际pH值显著高于ES8。根际pH值与根尖铝含量呈极显著负相关(R2=0.9321),与根相对伸长率呈极显著正相关(R2=0.8585),表明小麦通过提高根际pH值降低根尖铝含量,减轻铝毒害。根尖H+-ATPase活性随铝处理浓度升高而显著降低,100μmol·L-1Al处理24hET8和ES8根尖H+-ATPase活性分别为各自无铝处理的69.8%和60.0%,根尖H+-ATPase相对活性与根际pH值呈极显著负相关(R2=0.8319)。温度显著影响根的伸长,低温处理(9℃)根际pH值显著高于常温处理(25℃),而根尖铝含量却显著低于常温处理。表明小麦通过根尖H+-ATPas...

关 键 词:小麦  铝毒  根际pH  H+-ATPase  

Changes of Rhizosphere pH and the Relationship with Al-resistant of Different Al-Tolerant Wheat(Triticum aestivum L.)Under Al Stress
YANG Ye,GUO Zai-hua,YE Zhi-juan,GENG Ming-jian,WANG Wei,NING Da-wei,ZHAO Zhu-qing.Changes of Rhizosphere pH and the Relationship with Al-resistant of Different Al-Tolerant Wheat(Triticum aestivum L.)Under Al Stress[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2010,29(4).
Authors:YANG Ye  GUO Zai-hua  YE Zhi-juan  GENG Ming-jian  WANG Wei  NING Da-wei  ZHAO Zhu-qing
Institution:1.College of Resources and Environment/a>;Huazhong Agricultural University/a>;Wuhan 430070/a>;China/a>;2.Wuhan Vocational College of Software and Engineering/a>;Wuhan 430205/a>;China
Abstract:Al3+ is regarded as the greatest stress to plant and its activity is affected by environmental pH.Rhizosphere pH can be changed by H+ATPase which regulates the secretion of proton.To explain the relationship between changes of rhizosphere pH and Al-resistant of wheat (Triticum aestivum L.),the changes of rhizosphere pH and H+-ATPase activity of root apices of ET8(Al-resistant)and ES8(Al-sensitive)were investigated.The results were as follows:the rhizosphere pH rose with the prolonging of Al treatments,and t...
Keywords:wheat  aluminum toxicity  rhizosphere pH  H+-ATPase  
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