| Cre/loxp系统的构建及无选择标记转基因苹果植株的获得 |
| |
| 引用本文： | 李茂福,王媛花,王华,金万梅.Cre/loxp系统的构建及无选择标记转基因苹果植株的获得[J].园艺学报,2016,43(2):205-217. |
| |
| 作者姓名： | 李茂福 王媛花 王华 金万梅 |
| |
| 作者单位： | 北京市农林科学院林业果树研究所，北京市落叶果树工程技术研究中心，农业部华北地区园艺作物生物学与种质创制重点实验室，北京100093 |
| |
| 基金项目： | 国家高技术研究发展计划(‘863’)项目(2008AA10Z157)，北京市农林科学院科技创新能力建设专项(KJCX20140202)，国家自然科学青年基金项目(31201606)，科技创新能力建设专项(KJCX20140110)，北京市科委重大项目(D131100000113001) |
| |
| 摘 要： | 从‘霞晖8号’桃中克隆了一个定位于第4条染色体上的钾转运体基因PpeKUP5，该基因编码625个氨基酸，具有10个跨膜结构域；PpeKUP5主要在根部表达，其次是叶和茎中，花和果实中的表达量极低；ABA、重金属Cr和Zn处理均显著诱导其在桃幼苗各组织中的表达，其中Cr处理最为显著，高钾胁迫及重金属Cu处理显著降低了其在根部的表达水平；细菌互补试验表明PpeKUP5具有吸收外界K+（KCl或K2SO4）的功能，且在偏中性pH值条件下最为显著。本研究表明PpeKUP5是一个主导桃树根部K+吸收的钾转运体，并可能在桃树适应高钾及重金属Cr、Zn和Cu等胁迫处理和ABA 响应中起着重要作用。
|
| 关 键 词： | 平邑甜茶 烟草 Cre/loxp重组删除系统 无选择标记基因 |
Constructed the Cre/loxp Recombination Inducible Excision System and Obtained Marker-free Transgenic Apple Plants |
| |
| LI Mao-fu,WANG Yuan-hua,WANG Hua,JIN Wan-mei.Constructed the Cre/loxp Recombination Inducible Excision System and Obtained Marker-free Transgenic Apple Plants[J].Acta Horticulturae Sinica,2016,43(2):205-217. |
| |
| Authors: | LI Mao-fu WANG Yuan-hua WANG Hua JIN Wan-mei |
| |
| Institution: | Institute of Forestry and Pomology，Beijing Academy of Agriculture and Forestry Sciences，Beijing Engineering Research Center for Deciduous Fruit Trees，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture，Beijing 100093，China |
| |
| Abstract: | Used the Cre/loxp to establish inducible expression systems with site-specific recombination ability，to eliminate the selectable marker genes in transgenic plants specifically. Firstly，a binary expression vector PBI121-Cre-GUS with the Cre/loxp system was constructed to eliminate a marker gene from transgenic plants conveniently by digesting three intermediate vector and pBI121. Secondly，transgenic Malus hupehensis var. pinyiensis were obtained by using Agrobacterium mediated leaf disc method. These transgenic plants were induced by heat shock. Subsequently，the elimination efficiency was determined by GUS staining and PCR amplification. Finally，the transgenic plants without selectable marker gene were obtained. |
| |
| Keywords: | Malus hupehensis var pinyiensis tobacco Cre/loxp recombination excision system marker-free selectable gene |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
