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| 蒙古冰草Afa家族串联重复序列克隆及染色体定位 | |
| 引用本文： | 赵彦,窦全文,云锦凤,王俊杰.蒙古冰草Afa家族串联重复序列克隆及染色体定位[J].草地学报,2015,23(3):448-452. |
| 作者姓名： | 赵彦 窦全文 云锦凤 王俊杰 |
| 作者单位： | 1. 内蒙古农业大学生态环境学院, 内蒙古 呼和浩特 010018; 2. 草地资源教育部重点实验室, 内蒙古 呼和浩特 010018; 3. 中科院西北高原生物研究所, 青海 西宁 810008 |
| 基金项目： | 国家自然科学基金(31160479)(31260578)，内蒙古草业重大专项“优质牧草种质资源创新与产业化示范”(20120245)，National Nature Science Foundation of China(31260578 |
| 摘 要： | Afa家族串联重复序列因只出现在小麦及小麦族近缘属物种而得名,本研究从蒙古冰草中克隆得到一个Afa家族序列,长度为233 bp,命名为pAmAfa1,该序列在GENBANK中进行同源序列比对,结果表明该序列与大多数小麦族其他物种的Afa家族串联重复序列存在较高的相似性;系统进化分析表明,蒙古冰草pAmAfa1序列与大赖草pLrAfa3,pLrAfa5序列聚在一起,表明蒙古冰草P染色体组与大赖草的N、X染色体亲源关系较近。为了明确Afa家族串联重复序列在蒙古冰草染色体上的位置,双色荧光原位杂交技术被采用,以pAmAfa1为探针检测到杂交信号出现在染色体的末端或近端部的区域,每条染色体上都有杂交信号,表明Afa家族串联重复序列普遍存在于P染色体组中。 |
| 关 键 词： | 蒙古冰草 Afa家族串联重复序列 克隆 荧光原位杂交 |
| 收稿时间： | 2014-05-26 |
Isolating and location of Tandem Repetitive Afa-family Sequences from Mongolian Wheatgrass (Agropyron mongolicum Keng) |
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| ZHAO Yan,DOU Quan-wen,YUN Jin-feng,WANG Jun-jie.Isolating and location of Tandem Repetitive Afa-family Sequences from Mongolian Wheatgrass (Agropyron mongolicum Keng)[J].Acta Agrestia Sinica,2015,23(3):448-452. | |
| Authors: | ZHAO Yan DOU Quan-wen YUN Jin-feng WANG Jun-jie |
| Institution: | 1. Ecology and environment college of Inner Mongolia agricultural University (IMAU), Huhhot, Inner Mongolia 010019, China; 2. Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, Inner Mongolia 010019, China; 3. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China |
| Abstract: | Tandem repetitive Afa-family sequences are known to occur in the wheat and related species of Triticeae. A member of the Afa-family sequences isolated from Mongolian Wheatgrass (Agropyron mongolicum Keng) (2n=2x=14,PP) is 233 bp, named as pAmAfa1, which is most similar with other Afa-family sequences in Triticeae species. The phylogenic analysis results show that pAmAfa1 is clustered with pLrAfa3 and pLrAfa5, and the findings indicate that P genome has homology with N and X genomes. To know which the genome of Mongolian wheatgrass carries the Afa-family sequence; FISH is carried out using pAmAfa1 as the probe. The signals appear in the telomeric regions and subtelomeric regions of all chromosomes. This finding indicates that P genomes contain Afa-family repeats. |
| Keywords: | Mongolian Wheatgrass Tandem repetitive Afa-family sequences Molecular Cloning FISH |
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