| 一种改良的棉花总DNA提取方法 |
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| 引用本文： | 郭红媛,佘茂云.一种改良的棉花总DNA提取方法[J].山西农业科学,2009,37(2):3-5. |
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| 作者姓名： | 郭红媛 佘茂云 |
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| 作者单位： | 1. 山西农业大学农学院,山西太谷,030801
2. 中国农业科学院,北京,100081 |
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| 摘 要： | 为了获取高质量的棉花基因组DNA,进行了后续分子实验,通过对已有的几种DNA提取方法进行综合比较,寻找一套优化的适于棉花高质量DNA的提取方法。结果表明,优化的棉花基因组DNA提取方法所提取的DNA纯度高,无降解现象,适用于进行常规PCR扩增以及进行高精度要求的SSR扩增。
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| 关 键 词： | 总DNA 提取 CTAB SDS |
An Improved Total DNA Extraction Method |
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| GUO Hong-yuan,SHE Mao-yun.An Improved Total DNA Extraction Method[J].Journal of Shanxi Agricultural Sciences,2009,37(2):3-5. |
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| Authors: | GUO Hong-yuan SHE Mao-yun |
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| Institution: | GUO Hong-yuan, SHE Mao-yun ( 1. Agoronomy College ,Shartxi Agricultural University, Taigu 030801, China ; 2. Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | A new extract method of total DNA from plant was reported. The method combines CTAB method and SDS method. The total DNA obtained by the method showed to be more integral, pure and producing and suitable for PCR, Southern blotting, SSR and other molecular biology operation. |
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| Keywords: | CTAB SDS |
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