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苗药大果木姜子总黄酮与糖类成分含量测定
引用本文:刘刚,刘育辰,康雪佳,于福来,庞玉新,李霞,贾金燕.苗药大果木姜子总黄酮与糖类成分含量测定[J].安徽农业科学,2017,45(3).
作者姓名:刘刚  刘育辰  康雪佳  于福来  庞玉新  李霞  贾金燕
作者单位:贵阳中医学院,贵州贵阳550002;国家苗药工程技术研究中心,贵州贵阳550025;贵阳中医学院,贵州贵阳,550002;中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737;海南省艾纳香工程技术研究中心,海南儋州571737;贵州宏宇药业有限公司,贵州贵阳,550018
基金项目:贵州重大课题专项计划项目
摘    要:目的]建立测定大果木姜子中总黄酮、总糖、还原糖、多糖含量的方法。方法]以芦丁和葡萄糖为对照品,采用紫外分光光度计测定大果木姜子中总黄酮、总糖、还原糖、多糖的含量并进行方法学考察。结果]大果木姜子中总黄酮含量为(8.02±0.19)mg/g,总糖含量为(35.26±0.96)%,还原糖含量为(4.78±0.95)%,多糖含量为(30.53±0.69)%。结论]该方法快速、简便、准确,可以用于大果木姜子中总黄酮、总糖、还原糖、多糖的含量测定。

关 键 词:大果木姜子  总黄酮  总糖  还原糖  多糖  紫外分光光度法

Content Determination of Total Flavonoids and Sugar Components in Cinnamomum migao
Abstract:Objective] To establish the method for determination of total flavonoids, total sugar, reducing sugar, and polysaccharide in Cin-namomum migao. Method] Using rutin and glucose as control products, the contents of total flavonoids, total sugar, reducing sugar and pol-ysaccharide in C.migao were detected by UV spectrophotometer, and experiments were conducted to investigate whether the method is feasi-ble.Result] The contents of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao were (8.02 ±0.19) mg/g, (35.26 ±0.96)%, (4.78 ±0.95)%, (30.53 ±0.69)%, respectively.Conclusion] The method is rapid, simple, accurate, which is a-ble to accomplish the content determination of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao.
Keywords:Cinnamomum migao  Total flavonoids  Total sugar  Reducing sugar  Polysaccharide  UV
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