| 玉米QR-001/QS-001组合F_2群体SSR连锁图谱构建 |
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| 引用本文： | 宫源,;兰进好.玉米QR-001/QS-001组合F_2群体SSR连锁图谱构建[J].山东农业科学,2014(6):11-15. |
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| 作者姓名： | 宫源 ;兰进好 |
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| 作者单位： | [1]青岛农业大学农学与植物保护学院/青岛市主要农作物种质资源创新与应用重点实验室,山东青岛266109; [2]青岛农业大学农学与植物保护学院/青岛市主要农作物种质资源创新与应用重点实验室,山东青岛266109 |
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| 基金项目： | 山东省玉米育种与栽培技术企业重点实验室开放课题项目、青岛市科技计划基础研究项目[12-1-4-5-（6）-jch]、山东省自然科学基金（ZR2009DN014）、山东省现代农业产业技术体系创新团队项目（6210W3）共同资助. |
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| 摘 要： | 以自育的高抗玉米粗缩病自交系QR-001为母本、高感自交系QS-001为父本,配制了包含281个单株的F2分离群体为作图群体。选用在玉米基因组中均匀分布的1 028对SSR引物在双亲间进行多态性引物的筛选,共检测到110个可以鉴别F2群体各单株标记基因型的SSR多态性标记。借助QTL IciMapping Version 3.3软件构建了较好覆盖玉米10条染色体的分子标记连锁图谱,图谱总长度为1 343.2 cM,标记间平均距离为10.3 cM。其中1个分子标记(bnlg161)不与连锁群连锁。所建连锁群中有26.6%的SSR分子标记发生偏分离。该图谱的构建为玉米抗粗缩病QTL定位、分子标记辅助选择和分子设计抗病育种等研究奠定了基础。
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| 关 键 词： | 玉米 抗粗缩病种质 F2群体 SSR分子标记 连锁图谱 |
Construction of Genetic Linkage Map Based on SSR Marker in Maize F2 Population Derived from QR-001 and QS-001 Crossing |
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| Institution: | Gong Yuan, Lan Jinhao ( 1. College of Agronomy and Plant Protection, Qingdao Agricultural UniversitHQingdao Key Laboratory of Main Crop Germplasm Innovation and Utilization, Qingdao 266109, China; 2. Shandong Denghai Seeds Co. , Ltd./Shandong Provincial Key Laboratory of Corn Breeding and Cultivation Technology, Laizhou 261400, China) |
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| Abstract: | The inbred line QR-001 with high resistance to maize rough dwarf virus （ MRDV） as female parent , and the highly susceptible inbred line QS -001 as male parent , were used to produce a F2 segregation mapping population including 281 individuals .1 028 pairs of SSR primers , which distributed evenly though -out the genome in maize （ Zea mays L.） , were used for screening polymorphisms between the parental lines . And 110 polymorphic SSR markers were identified .The linkage group covering 10 chromosomes were construc-ted using the software QTL IciMapping Version 3.3.The total length of linkage group was 1 343.2 cM, and the even distance of each marker interval was 10 .3 cM.Only 1 molecular marker （ bnlg161 ） was not linked with the population .There were 26 .6%of all the SSR markers occurring segregation distortion .The linkage group was helpful for the MRDV QTL mapping , molecular marker-assisted selection and molecular design of resist-ance breeding . |
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| Keywords: | Maize Germplasm resistant to maize rough dwarf virus F2 population SSR molecular marker Genetic linkage map |
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