首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences
Authors:Birkenheuer Adam J  Breitschwerdt Edward B  Alleman A Rick  Pitulle Christian
Institution:Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606-1428, USA.
Abstract:OBJECTIVE: To determine whether Haemobartonella canis and Mycoplasma haemofelis (formerly known as H felis large form]) can be differentiated by use of comparative analysis of gene sequences. SAMPLE POPULATION: Blood samples obtained from 3 dogs infected with H canis and 2 cats infected with M haemofelis. PROCEDURE: The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained from H canis-infected dogs and M haemofelis-infected cats. The DNA sequences were subjected to comparative analysis. RESULTS: The 16S rDNA sequences of H canis and M haemofelis were nearly identical (homology of 99.3 to 99.7%). In contrast, RNase P gene sequences had a lower degree of sequence homology between the 2 organisms (94.3 to 95.5%). CONCLUSIONS AND CLINICAL RELEVANCE: Haemobartonella canis and M haemofelis are not identical organisms. Molecular differentiation of H canis and M haemofelis is more clearly evident by use of comparative analysis of RNase P gene sequences than by comparative analysis of 16S rDNA gene sequences.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号