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| 玉米品种SSR标记毛细管电泳荧光检测法与变性PAGE银染检测法的比较研究 | |
| 引用本文： | 易红梅,王凤格,赵久然,王璐,郭景伦,原亚萍.玉米品种SSR标记毛细管电泳荧光检测法与变性PAGE银染检测法的比较研究[J].华北农学报,2006,21(5):64-67. |
| 作者姓名： | 易红梅 王凤格 赵久然 王璐 郭景伦 原亚萍 |
| 作者单位： | 1. 北京市农林科学院玉米研究中心,北京,100089;首都师范大学,北京,100089 2. 北京市农林科学院玉米研究中心,北京,100089 3. 吉林大学,植物科学学院,吉林,长春,130062 |
| 基金项目： | 北京市自然科学基金 , 北京市科技新星计划项目 , 吉林省科技基金 |
| 摘 要： | 以192份玉米品种为材料,用7对SSR引物对毛细管电泳荧光检测和常规的变性PAGE银染检测两种SSR标记检测方法进行比较分析。结果表明,两种方法检测的SSR标记片段大小一致。毛细管电泳荧光检测法检测的结果更为精确、灵敏、高效,更适用于高通量材料的检测分析。对少量材料进行检测分析以及SSR标记的筛选时,使用常规的变性PAGE银染检测法更经济适用。 |
| 关 键 词： | 玉米 SSR 毛细管电泳 银染 |
| 文章编号： | 1000-7091（2006）05-0064-04 |
| 收稿时间： | 2006-04-20 |
| 修稿时间： | 2006-04-20 |
Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method |
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| YI Hong-mei,WANG Feng-ge,ZHAO Jiu-ran,WANG Lu,GUO Jing-lun,YUAN Ya-ping.Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method[J].Acta Agriculturae Boreali-Sinica,2006,21(5):64-67. | |
| Authors: | YI Hong-mei WANG Feng-ge ZHAO Jiu-ran WANG Lu GUO Jing-lun YUAN Ya-ping |
| Institution: | 1.Maize Reasereh Center, Beijing Academy Agricultural and Forestry Science, Beijing 100089, China; 2. Capital Normal University, Beijing 100089, China; 3. Department of Plant Science, Jilin University, Changehun 130062,China |
| Abstract: | Seven SSR primers and 192 maize hybrid were used to comparatively analyze SSR markers between capillary electrophoresis with fluorescence detection and denaturing PAGE(polyacrylamide gel electrophoresis) silver-staining detection.The results indicated that SSR markers fragment sizes explored by two decetion systems were relatively similar.Capillary electrophoresis with fluorescence detection has the advantages of high accuracy,sensitiveness,cost effectiveness and high-throught.Therefore,the capillary electrophoresis with fluorescence detection system is suggested to be suitable for scaning and analyzing large-scale material.While denaturing PAGE silver-staining detection would be used in examaining a few samples,especially in screening SSR markers. |
| Keywords: | Maize SSR Capillary electrophoresis Silver-staining |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |