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| 马铃薯花粉离体培养影响因子分析 | |
| 引用本文： | 吴旺泽,彭晓莉,王蒂,刘小平.马铃薯花粉离体培养影响因子分析[J].甘肃农业大学学报,2009,44(5). |
| 作者姓名： | 吴旺泽 彭晓莉 王蒂 刘小平 |
| 作者单位： | 1. 安徽省农业科学院作物研究所,安徽,合肥,230031甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070 2. 合肥工业大学生物与食品工程学院,安徽,合肥,230009 3. 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070 4. 安徽省农业科学院作物研究所,安徽,合肥,230031 |
| 基金项目： | 国家基础研究发展规划"973"项目，甘肃省农业厅项目 |
| 摘 要： | 以马铃薯优良四倍体栽培种‘唐113’、‘陇薯3号’和‘甘农薯1号’为材料,对不同发育时期的花粉进行了离体悬浮培养.结果表明,培养前将材料于4~7℃低温下处理7 d或0.3 mol.L-1甘露醇中饥饿处理3 d均取得了较好的培养效果,花粉分裂频率分别达到13.2%和13.6%.马铃薯单核期花粉具有孢子体发育的巨大潜力,分裂频率高于其他发育时期的花粉,其中‘唐113’脱分化率达4.1%,是马铃薯花粉诱导愈伤组织和再生植株培养的适宜时期;不同品种花粉离体的脱分化频率表明马铃薯花粉培养存在基因性依赖. |
| 关 键 词： | 马铃薯 花粉离体培养 预处理 发育途径 |
Analysis of factors affecting pollen culture in vitro of Solanum tuberosum |
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| WU Wang-ze,PENG Xiao-li,WANG Di,LIU Xiao-ping.Analysis of factors affecting pollen culture in vitro of Solanum tuberosum[J].Journal of Gansu Agricultural University,2009,44(5). | |
| Authors: | WU Wang-ze PENG Xiao-li WANG Di LIU Xiao-ping |
| Institution: | WU Wang-ze1,2,3,PENG Xiao-li4,WANG Di2,LIU Xiao-ping1(1.Crop Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China,2.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,3.Gansu Key Lab of Crop Improvement & Germplasm Enhancement,4.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China) |
| Abstract: | Three potato varieties,'Tang 113','Long Shu No. 3' and 'Gannong Shu No. L',were em-ployed as raw material for pollen culture in vitro. The results indicated that pretreatment at 4～7 ℃for 7 days or mannitol at 0. 3 mol ·L~(-1) for 3 days enhanced the division frequency of pollen. The division fre-quency reached up to 13. 2 % and 13. 6 %. Pollen in monokaryotic period had a better potential for develop-ment of microspore,and had a higher division frequency. Pollen culture in vitro of different variety indica-ted that dedifferentiation frequency referred genotype of potato cultivar. |
| Keywords: | Solanum tuberosum pollen culture in vitro pretreatment development pathway |
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