三聚氰胺人工抗原合成与鉴定 |
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引用本文: | 王旻子,应永飞,龚云飞,方结红,陈宗伦,张明洲.三聚氰胺人工抗原合成与鉴定[J].安徽农学通报,2011,17(23):43-45. |
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作者姓名: | 王旻子 应永飞 龚云飞 方结红 陈宗伦 张明洲 |
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作者单位: | 1. 杭州迪恩科技有限公司,浙江杭州,310013 2. 浙江省畜产品质量安全检测中心,浙江杭州,310020 3. 中国计量学院生命科学学院,浙江省生物计量与检验检疫技术重点实验室,浙江杭州310018 |
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基金项目: | 浙江省重大科技专项,浙江省重点科技创新团队 |
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摘 要: | 采用戊二醛法与甲醛树脂法分别将三聚氰胺(Melamine,Mel)偶联到牛血清白蛋白(BSA)与鸡卵清蛋白上,合成免疫原三聚氰胺-牛血清白蛋白(Mel-BSA)与包被原三聚氰胺-鸡卵清蛋白(Mel-OVA)。通过快速凝胶液相层析(FPLC)纯化后,合成抗原进行紫外扫描、红外扫描和SDS-PAGE电泳鉴定。结果表明三聚氰胺已偶联到载体蛋白,为进一步制备三聚氰胺的特异性抗体与建立三聚氰胺免疫检测方法奠定了基础。
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关 键 词: | 三聚氰胺 人工抗原 合成 鉴定 |
Preparation and Indentification of Artificial Antigen of Melamine |
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Institution: | Wang Minzi et al.(2 Hangzhou DNA Sci-tech Co.,Ltd.,Hangzhou 310013,China) |
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Abstract: | Though the method of glutaraldehyde,the melamine was coupled to the carrier protein for synthesizing the artificial antigen.Melamine was linked to bovine serum ovabumin(BSA) to generate immunogenic antigen(Mel-BSA) and was also linked to ovabumin(OVA) by melamino-formaldehyde resin method to generate coating antigen Mel-OVA.Artificial antigen was purified by FPLC,identified by UV and IR spectroscopies as well as SDS-PAGE.The synthesis of melamine artificial antigen make it possible to establish a base of the generation specific antibody and development of immunoassay of melamine. |
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Keywords: | Melamine Artificial Antigen Preparation Indentification |
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