The effect of in planta TIBA and proline treatment on somatic embryogenesis of sugar beet (Beta vulgaris L.) |
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| Authors: | Behrouz Ehsani Moghaddam Mahmoud Mesbah Nasrin Yavari |
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| Institution: | (1) Sugar Beet Seed Institute, P.O. Box 31585-4114, Karadj, Iran |
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| Abstract: | The effect of in planta TIBA and L-proline onin vitro seedlings and cell culture of sugar beet was investigated. Sterilized seeds were grownin vitro on 1/2 MS medium supplemented with 0 or3 mg/l TIBA. Calli obtained on young leaves cultured on MS medium containing 1 mg/l
BAP, were used for the initiation of cell suspension cultures using MS basal composition supplemented with 0 or 50 mM proline.
Aliquots of 1 ml from cell suspension culture were inoculated onto the first somatic embryo induction MS medium containing
TIBA 0.5 mg/l, BAP 1.0 mg/l, and proline at 0 or 50 mM. After three weeks of culture, embryogenic calli were transferred to
the second embryo induction medium supplemented with NAA and BAP at 0.2 and 0.5 mg/l, respectively. The frequency of somatic
embryos of calli obtained from in plantaTIBA together with proline treatments on average was20 which was higher than that of the other treatments.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | cell culture proline sugar beet TIBA |
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