| 云南省瓜类细菌性果斑病的鉴定 |
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| 引用本文： | 朱晓媛,王文成,徐烨,刘雅婷,郑元仙,江常杰,王金龙.云南省瓜类细菌性果斑病的鉴定[J].云南农业大学学报,2013,28(2):175-180. |
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| 作者姓名： | 朱晓媛 王文成 徐烨 刘雅婷 郑元仙 江常杰 王金龙 |
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| 作者单位： | 1云南农业大学 农学与生物技术学院，云南 昆明 650201；2云南大学 生态学与地植物学研究所,云南 昆明 650091；3经济林木种质改良与资源综合利用湖北省重点实验室, 湖北 黄冈 438000；4云南省烟草公司 临沧市公司，云南 临沧 677099 |
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| 摘 要： | 瓜类细菌性果斑病(BFB)是严重危害葫芦科作物的细菌病害。2009—2010年，通过田间调查，发现云南西双版纳州和普洱市部分地区的西瓜(Citrullus lanatus)和甜瓜（Cucumis melo）上出现疑似瓜类细菌性果斑病病害发生。通过菌株分离纯化、菌体形态和菌落形态特征、革兰氏染色、致病性测定、微生物鉴定系统测定、分子鉴定等方法进行诊断鉴定，结果表明获得的yn2和yn3菌株与西瓜噬酸菌(Acidovorax citrulli) 的特征一致。确定了云南省已有瓜类细菌性果斑病分布，为云南省首次报道。
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| 关 键 词： | 瓜类细菌性果斑病 噬酸菌属燕麦种西瓜亚种 形态特征 微生物鉴定系统 分子鉴定 |
Identification of Bacterial Fruit Blotch of Melons Found in Yunnan Province |
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| ZHU Xiao-yuan,WANG Wen-cheng,XU Ye,LIU Ya-ting,ZHENG Yuan-xian,JIANG Chang-jie,WANG Jin-long.Identification of Bacterial Fruit Blotch of Melons Found in Yunnan Province[J].Journal of Yunnan Agricultural University,2013,28(2):175-180. |
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| Authors: | ZHU Xiao-yuan WANG Wen-cheng XU Ye LIU Ya-ting ZHENG Yuan-xian JIANG Chang-jie WANG Jin-long |
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| Institution: | 1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201，China; 2Ecology and Geobotany Institute, Yunnan University, Kunming 650201，China;3Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources in Hubei Province Key Laboratory,Huanggang 438000, China;4China National Tobacco Corporation Yunnan Province Company Lincang Branch，Lincang 677099，China |
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| Abstract: | Bacterial fruit blotch of melons causes serious damages on the crops of cucurbits worldwide. From 2009 to 2010, suspected samples of melons were found from watermelon (Citrullus lanatus) and muskmelon (Cucumis melo) in Xishuangbanna and Puer prefectures of Yunnan Province by field survey. Two isolates, yn2 and yn3，with the same characteristic of Acidovorax citrulli were identified through pathogen isolation and purification, mycelia morphology and colony shape observation, gram staining, pathogenicity test, biolog automated microbial identification and susceptibility system, and molecular identification. It was confirmed that bacterial fruit blotch of melons occurred in Yunnan Province. This is the first report for this disease in Yunnan Province. |
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| Keywords: | bacterial fruit blotch of melons(BFB) Acidovorax citrulli morphological characteristic microbial identification system molecular identification |
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