| 二甲基亚砜溶剂用于制霉菌素效价测定 |
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| 引用本文： | 于海兵,谢卫国,褚志刚,阮琼芳,李华文,李敏,丁元林,孔丹莉,姚大纯.二甲基亚砜溶剂用于制霉菌素效价测定[J].湛江医学院学报,2017,35(3). |
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| 作者姓名： | 于海兵 谢卫国 褚志刚 阮琼芳 李华文 李敏 丁元林 孔丹莉 姚大纯 |
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| 作者单位： | 1. 广东医科大学公共卫生学院,广东东莞,523808;2. 武汉大学同仁医院烧伤研究所,武汉,430060;3. 广东医科大学公共卫生学院,广东东莞523808;武汉大学同仁医院烧伤研究所,武汉430060 |
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| 基金项目： | 国家自然科学基金(No.81273166 and 81501667)This study was financially supported by The National Natural Science Foundation of China;Project #:81273166 and 81501667，The Wuhan Science and Technology Innovation Project #:201507040401021，The International Technology Cooperation Projects of Dongguan (20135081520017).The Features Innovative Projects of Key Platform and Major Scientific Research Project of Universities in Guangdong Province #2015KTSCX048，The Technology Plan Projects of Medical and Health Science in Dongguan City#201605101290 |
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| 摘 要： | 目的 探讨二甲基亚砜(DMSO)代替N,N-二甲基甲酰胺(DMF)用于制霉菌素效价测定的可行性.方法 制霉菌素分别用DMSO和DMF溶解,采用美国药典中抗生素-微生物测定法检测制霉菌素效价.结果 DMF和DMSO均有较好的准确性、精密度、特异性、线性范围(10.0-40.0 U/mL)、检出限(2.0 U/mL)和稳定性,且DMSO方法的灵敏度、定量下限(LOQ,3.0 U/mL)优于DMF.结论 鉴于DMSO毒性明显低于DMF,且DMSO方法的分析性能特征与DMF法相当甚或更好,故DMSO可代替DMF作为溶剂用于制霉菌素活性测定.
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| 关 键 词： | 制霉菌素 效价测定 二甲基亚砜 N N-二甲基甲酰胺 |
Development and validation of nystatin potency assay using solvent dimethyl sulfoxide |
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| YU Hai-bing,XIE Wei-guo,CHU Zhi-gang,RUAN Qiong-fang,LI Hua-wen,LI Min,DING Yuan-ling,KONG Dan-li,Paul Yao.Development and validation of nystatin potency assay using solvent dimethyl sulfoxide[J].Journal of Guangdong Medical College,2017,35(3). |
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| Authors: | YU Hai-bing XIE Wei-guo CHU Zhi-gang RUAN Qiong-fang LI Hua-wen LI Min DING Yuan-ling KONG Dan-li Paul Yao |
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| Abstract: | Objective To investigate the feasibility of dimethyl sulfoxide (DMSO) instead of N,N-dimethyl formamide (DMF) as a solvent for the nystatin potency assay.Methods Nystatin was dissolved in DMSO or DMF,and its potency was determined using antibiotic microbial assay of the United States Pharmacopeia (USP).Results Both DMF and DMSO methods had satisfied accuracy,precision,specificity,linear range (10.0-40.0 U/mL),limit of detection (2.0 U/mL) and robustness.Meanwhile,DMSO method has higher sensitivity and lower limit of quantitation (LOQ,3.0 U/mL) than DMF method.Conclusion Since DMSO has much less toxicity than DMF,and DMSO method has equivalent or even better analytical performance characteristics than DMF method,DMSO may be a good candidate to replace DMF as the solvent for the determination of nystatin activity. |
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| Keywords: | nystatin potency assay dimethyl sulfoxide N N-dimethyl formamide |
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