| 柑橘绿霉病菌拮抗放线菌MY-5的筛选与鉴定 |
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| 引用本文： | 李辉,耿鹏,郝卫宁,胡美英.柑橘绿霉病菌拮抗放线菌MY-5的筛选与鉴定[J].中国农学通报,2010,26(19):280-284. |
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| 作者姓名： | 李辉 耿鹏 郝卫宁 胡美英 |
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| 作者单位： | 华南农业大学天然农药与化学生物学教育部重点实验室,广州,510642 |
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| 基金项目： | 国家自然科学基金项目，广东省科技计划项目 |
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| 摘 要： | 从广东省各地区水果园和蔬菜地采集土壤23份，采用稀释平板法分离获得放线菌212株。以柑橘绿霉病菌为目标菌，采用抑菌带测定法，筛选获得9株具有拮抗活性的菌株，其中菌株MY-5拮抗活性最强，抑菌带宽达18.67mm，同时对其它13种果蔬采后病害也有较强的拮抗作用。通过对菌株MY-5形态观察、培养特征、生理生化反应和16SrDNA鉴定，该菌株为链霉菌属吸水类群（Streptomyces hygroscopicus）。
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| 关 键 词： | 巴西橡胶树 巴西橡胶树 K+通道基因 HbKCO1 高效表达 |
| 收稿时间： | 2010/4/28 0:00:00 |
| 修稿时间： | 6/5/2010 12:00:00 AM |
Screening and identification of Actinomyces MY-5 antagonistic against Penicillium digitatum Sacc. |
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| Li Hui,Ge Peng,Hao Weining,Hu Meiying.Screening and identification of Actinomyces MY-5 antagonistic against Penicillium digitatum Sacc.[J].Chinese Agricultural Science Bulletin,2010,26(19):280-284. |
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| Authors: | Li Hui Ge Peng Hao Weining Hu Meiying |
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| Institution: | (Key Laboratory of Pesticide and Chemical Biology , Ministry of Education , South China Agricultural University , Guangzhou 510642 ) |
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| Abstract: | The aim of the study to screen anti-microorganism and find new active metabolites for control postharvest disease of fruit and vegetables. Two hundred and twenty-one actinomycetes strains were obtained from twenty-three soil samples collected from fruit orchards and vegetable fields in different parts of Guangdong Province. 9 strains showed antagonistic activities against Penicillium digitatum Sacc. Among them , strain MY-5 exhibited the strongest antibiosis capacity with an inhibitory zone of 18.67 mm. Strain MY-5 was identified as Streptomyces hygroscopicus according to its 16S rDNA sequence analysis , and the morphological , physiological and biochemical characteristics. |
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| Keywords: | Penicillium digitatum Sacc screening antagonistic effect actinomycetes identification orthogonal experiment |
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