| 一种简便、快速检测鸡毒支原体的PCR方法 |
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| 引用本文： | 林居纯,曾振灵,蒋红霞,陈杖榴.一种简便、快速检测鸡毒支原体的PCR方法[J].畜禽业,2006(12):44-47. |
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| 作者姓名： | 林居纯 曾振灵 蒋红霞 陈杖榴 |
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| 作者单位： | 华南农业大学广东省兽药研制与安全评价重点实验室,广东,广州,510642 |
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| 摘 要： | 鸡毒支原体(MG)是造成鸡慢性呼吸道疾病的主要病原菌。通常采用血清学方法诊断该病,由于存在非特异性交叉反应,这给临床诊断带来困难。为解决MG快速诊断问题,该研究根据已发表的鸡毒支原体种特异性序列FMG-2合成一对引物(MG1,MG2),用PCR方法检测MG。结果表明,该PCR方法对MG能特异性扩增732bp目的片断,而对照菌(大肠杆菌、金黄色葡萄球菌等)却不能扩增出目的片段。PCR产物经测序,其序列与Genbank序列同源性为98.86%。用PCR方法检测60份感染样本,其阳性检出率为80%,而用传统的分离培养方法检出率仅为50%。
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| 关 键 词： | 鸡毒支原体 PCR 检测 |
| 文章编号： | 1008-0414(2006)06(下)-0044-04 |
| 修稿时间： | 2006年4月18日 |
A Rapid Method Of The Polymerase Chain Reaction (PCR) for Detection Of Mycoplasma Gallisepticum |
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| LIN Ju-chun,ZENG Zhen-ling,JIANG Hong-xia,CHEN Zhang-liu.A Rapid Method Of The Polymerase Chain Reaction (PCR) for Detection Of Mycoplasma Gallisepticum[J].Livestock and Poultry Industry,2006(12):44-47. |
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| Authors: | LIN Ju-chun ZENG Zhen-ling JIANG Hong-xia CHEN Zhang-liu |
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| Abstract: | Mycoplasma gallisepticum(MG) is the etiologic agent of avian chronic respiratory disease. Traditional serologic test for the diagnosis of the disease has encountered with cross-reaction. Therefore, it is difficult to diagnose MG infection with serologic methods. In order to solve this problem ,a PCR method based the reported DNA sequence of FMG-2 to detect MG was developed. A 732-bp expected fragment was amplified from MG DNA, but it was not amplified from other bacteria(including E.coli,S.aureus,MS,et al ). Products of PCR from a reference strain and an isolate were sequenced .In comparison with the sequences in GenBank, the homology was 98.86%.Among the 60 clinical samples, the positive ratio was 80% for PCR, but the positive ratio was only 50% for isolation method. |
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| Keywords: | PCR |
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