| 普通西瓜3个变种45S rDNA和5S rDNA的染色体定位 |
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| 引用本文： | 江姣,张海英,郭绍贵,任毅,孙宏贺,宫国义,许勇,赵泓.普通西瓜3个变种45S rDNA和5S rDNA的染色体定位[J].果树学报,2012(5):764-769,964. |
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| 作者姓名： | 江姣 张海英 郭绍贵 任毅 孙宏贺 宫国义 许勇 赵泓 |
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| 作者单位： | 首都师范大学;北京市农林科学院蔬菜研究中心;北京农学院 |
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| 基金项目： | 科技部国际合作项目(2010DFB33740);国家现代西甜瓜产业技术体系(CARS-26);国家自然科学基金项目(31171980);北京市自然科学基金重大项目(5100001);育种平台三期(D111100001311002) |
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| 摘 要： | 【目的】为了探明普通西瓜种(Citrullus lanatus)3个变种间的亲缘关系和进化与驯化过程,【方法】利用双色荧光原位杂交技术(fluorescence in situ hybridization,FISH),将核糖体45S rDNA和5S rDNA在11份栽培变种(Citrulluslanatus subsp.vulgaris)、2份Egusi变种(C.lanatus subsp.mucosospermus)和3份Citroides变种(C.lanatus subsp.lana-tus)有丝分裂中期染色体上进行了定位研究。11份栽培变种西瓜和2份Egusi类型西瓜都含有2对45S rDNA位点和1对5S rDNA位点,3份Citroides变种都含有1对45S rDNA位点和2对5S rDNA位点。【结果】结果表明,普通西瓜种3个变种内部各基因型间具有相同的45S rDNA和5S rDNA分布模式,栽培变种和Egusi变种具有相同的分布模式,但它们均与Citroides变种存在明显差异,说明栽培变种与Egusi变种之间的亲缘关系更近一些。【结论】rDNA序列分布分析为在分子细胞学水平开展栽培西瓜的进化与驯化分析提供了证据。
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| 关 键 词： | 普通西瓜 荧光原位杂交(FISH) rDNA BAC文库 |
Chromosomal polymorphism of ribosomal genes in the genus watermelon |
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| JIANG Jiao,ZHANG Hai-ying,GUO Shao-gui,REN Yi,SUN Hong-he,GONG Guo-yi,XU Yong,ZHAO Hong.Chromosomal polymorphism of ribosomal genes in the genus watermelon[J].Journal of Fruit Science,2012(5):764-769,964. |
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| Authors: | JIANG Jiao ZHANG Hai-ying GUO Shao-gui REN Yi SUN Hong-he GONG Guo-yi XU Yong ZHAO Hong |
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| Institution: | 1Capital Normal University,Beijing 100048 China;2Biejing Vegetable Research Center,Beijing 100097 China;3Beijing University of Agriculture,Biejing 102206 China) |
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| Abstract: | 【Objective】The objective of the study is to investigate the genetic relationship,the process of evolution and domestication in watermelon.【Method】Both 45S rDNA and 5S rDNA were used as probes in the double fluorescence in situ hybridization(FISH) to be localized on chromosomes in 16 genotypes representing three subspecies.There are two pairs of 45S rDNA loci and one pair 5S rDNA locus in C.lanatus subsp.Vulgaris and subsp.Mucosospermus.C.lanatus subsp.lanatus possess one pair 45S rDNA locus and two pairs of 5S rDNA loci.【Result】The result showed that genotypes belonging to one subspecies have similar rDNA distribution pattern,and there was no significant differences between C.lanatus subsp.Vulgaris and C.lanatus subsp.Mucosospermus.C.lanatus subsp.lanatus is quiet different from the other two subspecies in the number and position of rDNAs sites.It is suggested that the distance between C.lanatus subsp.Vulgaris and C.lanatus subsp.Mucosospermus are closer than that of C.lanatus subsp.Lanatus.【Conclusion】The distribution analysis of rDNA sequences provides evidence in evolution and domestication for cultivated watermelon in molecular biology level. |
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| Keywords: | Watermelon Fluorescence in situ hybridization rDNA BACs |
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