| 外生菌根真菌彩色豆马勃优良菌株营养源的研究* |
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| 引用本文： | 花晓梅.外生菌根真菌彩色豆马勃优良菌株营养源的研究*[J].林业科学研究,1995,8(6):597-604. |
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| 作者姓名： | 花晓梅 |
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| 作者单位： | 中国林业科学研究院林业研究所 |
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| 基金项目： | “八五”国家科技攻关项目 |
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| 摘 要： | 通过Ｐ．ｔ．优良菌株９１０９在不同培养基上生长速度、培养特性及其生长规律的研究，筛选出生长最佳培养基作为基础培养基，进行Ｃ、Ｎ营养研究，试验结果表明：Ｐ．ｔ．９１０９不仅能利用单糖、低聚糖和无机Ｎ等速效Ｃ、Ｎ源，而且能利用多糖、多元醇和有机Ｎ等长效Ｃ、Ｎ源。其菌丝生长最适Ｃ源是玉米粉和甘露糖；最适Ｎ源是蛋白胨和氯化铵。在无机Ｎ利用中，对硝态Ｎ和铵态Ｎ的利用无显著差异。应用Ｌ１６（４５）正交设计确定了Ｐ．ｔ．９１０９菌丝生长的最佳营养配方，菌丝增殖高达８６０倍，不但提高菌丝生物产量，而且节省了原料，降低了成本，为改进Ｐ．ｔ．菌剂生产配方和研制新型菌剂提供了最佳营养条件。
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| 关 键 词： | 彩色豆马勃，培养基，C源，N源 |
| 收稿时间： | 1995/2/25 0:00:00 |
Nurtitional Source Study for Super-srtain of Ectomycorrhizal Fungus,Pisolithus tinctorius |
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| Hua Xiaomei.Nurtitional Source Study for Super-srtain of Ectomycorrhizal Fungus,Pisolithus tinctorius[J].Forest Research,1995,8(6):597-604. |
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| Authors: | Hua Xiaomei |
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| Institution: | the Research Institute of Forestry, CAF Beijing 100091 |
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| Abstract: | Through the study on growth rate,culture characteristics and growth rules
ofPisolithus tinctorius on different medium,the optimal medium was selected as the basic
medi-um for its carbon and nitrigen sources study. The results show that Pt has a wide range
ofnutritional sources for metabolization,not only can it use
monosaccharides,disaccharides,andinorganic nitrogen, but also it can use polysaccharides,
polyol and organic nitrogen. Cornmeal and mannitol are its optimal carbon sources, peptone
and NH4Cl the optimal nitrogensources for its mycelial growth. No obvious difference was
noted when amine and nitrateforms of inorganic nitrogen were utilized by the fungus. And the
optimum formulae of nutri-tion for its mycelial growth are obtained through orthogonal
experiment L16(4 5).Through us-ing the formulae,the biomass of the fungal mycelium can
astonishingly proliferate 860 times,which surpass that of the former report. Therefore,the
research provided the theory base toimprove the formula and the technology for producing the
ectomycorrhizal inocula of the fun-gus. |
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| Keywords: | Pisolithus tinctorius medium carbon source nitrogen sources |
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