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稻草秸秆栽培金针菇基质降解特性研究
引用本文:高金权,刘朝贵,李成琼.稻草秸秆栽培金针菇基质降解特性研究[J].中国农学通报,2005,21(12):260-264.
作者姓名:高金权  刘朝贵  李成琼
作者单位:1. 西南大学园艺园林学院,重庆,400716
2. 重庆市蔬菜学重点实验室,重庆,400716
基金项目:国家科技部重大项目“区域生态环境安全及生态经济技术系统重建关键技术研究与示范”(2004BA604A05).
摘    要:对稻草秸秆栽培金针菇(Flammulina velutipes)不同生长阶段,基质木质纤维成分的变化和与 基质主要成分降解相关的几种胞外酶的变化规律进行了研究。结果表明:纤维素和半纤维素的降解 主要集中在生殖生长期,木质素在营养生长期降解迅速;与纤维素降解相关的两种酶活性从培养初 期到结束一直保持比较平稳的趋势;与木质素降解相关酶在菌株培养初期活性较高,木聚糖酶活性 初期活性很低,在子实体形成期一直保持较高水平,淀粉酶活性高峰出现在初期,蛋白酶活性在初期 和子实体形成期较高,但整体上酶活性变化较为平稳。

关 键 词:稻草秸秆  金针菇  基质降解  胞外酶
收稿时间:2005-07-27
修稿时间:2005-07-27

Study on The Degradation of Rice Straw by Flammulina velutipes
Gao Jinquan,Liu Chaogui,Li Chengqiong.Study on The Degradation of Rice Straw by Flammulina velutipes[J].Chinese Agricultural Science Bulletin,2005,21(12):260-264.
Authors:Gao Jinquan  Liu Chaogui  Li Chengqiong
Institution:Southwest University, Key Lab in Vegetable Research of Chongqing, Chongqing 400716
Abstract:The strains of Flammulina velutipes are cultured with rice straw. The change of lignocellulose in medium and the transformational law of extracellular enzyme activity related to the degradation of major medium constituent at different culture stages are studied. Results show that the degradation of cellulose and hemicellulose are occurred in reproductive period primarily and the degradation of lignin is expediter in vegetative growth period. Enzymes related to the degradation of cellulose are more stable from cultivation prophase to the end. Enzymes related to the degradation of lignin are much higher at cultivation prophase. The activity of xylanase is lower and is higher at the phase of fruiting body occurred. The activity peak of amylase occurred in cultivation prophase. The activity of proteases are higher at cultivation prophase and the phase of fruiting body occurred,however,the activity is more stable.
Keywords:Rice straw  Flammulina velutipes  Degradation of medium  Extracellular enzyme
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