| 板蓝根多糖对体外培养鸡脾脏淋巴细胞蛋白激酶C活性的影响 |
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| 引用本文： | 刘俊玲,张元元,崔保安,张红英,王学兵.板蓝根多糖对体外培养鸡脾脏淋巴细胞蛋白激酶C活性的影响[J].中国农学通报,2011,27(11):15-19. |
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| 作者姓名： | 刘俊玲 张元元 崔保安 张红英 王学兵 |
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| 作者单位： | 河南农业大学牧医工程学院; |
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| 基金项目： | 国家博士点基金项目“板蓝根多糖对畜禽免疫系统信号转导影响研究”(20070466001); 郑州市创新型科技人才队伍建设工程资助项目(10CXTD148) |
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| 摘 要： | 采用水提醇沉法提取板蓝根多糖，应用蒽酮-硫酸法进行多糖含量测定。体外培养鸡脾脏淋巴细胞，用固相夹心酶联免疫吸附试验（ELISA）测定蛋白激酶c(PKC)活性，分析板蓝根多糖对鸡脾脏淋巴细胞PKC活性的影响。结果显示，加入板蓝根多糖组（100mg/ml）与空白对照组的PKC的活性有显著差异(P<0.05)，表明板蓝根多糖能引起鸡脾脏淋巴细胞蛋白激酶c活性明显升高。
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| 关 键 词： | 分子标记 分子标记 |
| 收稿时间： | 2010/12/7 0:00:00 |
| 修稿时间： | 2011/1/12 0:00:00 |
Effect of Indigowoad Root Polysaccharides on The Activity of Protein kinase C of Spleen lymphocytes in Chicken |
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| Liu Junling,Zhang Yuanyuan,Cui Baoan,Zhang Hongying,Wang Xuebing.Effect of Indigowoad Root Polysaccharides on The Activity of Protein kinase C of Spleen lymphocytes in Chicken[J].Chinese Agricultural Science Bulletin,2011,27(11):15-19. |
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| Authors: | Liu Junling Zhang Yuanyuan Cui Baoan Zhang Hongying Wang Xuebing |
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| Institution: | Liu Junling,Zhang Yuanyuan,Cui Baoan,Zhang Hongying,Wang Xuebing(Engineering College of Animal and Veterinary Science,Henan Agricultural University,Zhengzhou 450002) |
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| Abstract: | Indigowoad Root Polysaccharides(IRPS) was extracted by experimental boil alcohol extracti,using Anthrone-Sulfuric Acid Method for the Determination of Polysaccharides.Then cultivated Chicken Spleen lymphocytes in vitro to detect the Activity of Protein Kinase C(PKC) using Solid-Phase Sandwich Enzyme-Linked Immunosorbent Assay(ELISA) techniques and analyzed the effect of Indigowoad Root Polysaccharides on the Activity of Protein kinase C of Spleen lymphocytes in Chicken.The results showed that IRPS(100 mg/mL... |
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| Keywords: | iRPS chicken spleen lymphocytes protein kinasec |
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