| 番茄ACC氧化酶基因cDNA的克隆及三种植物表达载体构建 |
| |
| 引用本文： | 符秀梅,朱红林,周秀,李小靖,陈银华.番茄ACC氧化酶基因cDNA的克隆及三种植物表达载体构建[J].华北农学报,2009,24(Z2). |
| |
| 作者姓名： | 符秀梅 朱红林 周秀 李小靖 陈银华 |
| |
| 作者单位： | 1. 海南大学农学院,海南,海口,570228
2. 海南大学教育部热带生物资源重点实验室,海南,海口,570228;海南大学农学院,海南,海口,570228 |
| |
| 摘 要： | 乙烯在植物生长发育过程中发挥着重要的作用.ACC氧化酶是乙烯生物合成途径的限速酶.通过反义基因工程调控ACC氧化酶基因的表达,进而起到调控乙烯的生成是乙烯研究的主要途径.本研究采用RT-PCR方法获得番茄ACC氧化酶基因cDNA序列1 018 bp,构建该基因的正义、反义、RNAi表达载体,通过电转化法导入农杆菌LBA4404中.
|
| 关 键 词： | 番茄 ACO基因 表达载体构建 |
Cloning of ACC Oxidase Gene cDNA from Tomato and Construction of Three Plant Expression Vectors |
| |
| FU Xiu-mei,ZHU Hong-lin,ZHOU Xiu,LI Xiao-jing,CHEN Yin-hua.Cloning of ACC Oxidase Gene cDNA from Tomato and Construction of Three Plant Expression Vectors[J].Acta Agriculturae Boreali-Sinica,2009,24(Z2). |
| |
| Authors: | FU Xiu-mei ZHU Hong-lin ZHOU Xiu LI Xiao-jing CHEN Yin-hua |
| |
| Institution: | FU Xiu-mei2,ZHU Hong-lin2,ZHOU Xiu2,LI Xiao-jing2,CHEN Yin-hua1,2(1.Key Laboratory for Tropical Biological Resources,Ministry of Education,Haikou 570228,China,2.College of Agronomy,Hainan University,China) |
| |
| Abstract: | Ethylene, a phytohormone, plays an extensive role in plant growth and development. The enzyme ACC oxidase,catalysing the last step in the biosynthesis of the plant hormone ethylene, is the key enzyme in the course. The main research of ethylene is to control the respiration amount through the method of antisense-RNA. In this research,a cDNA fragment of 1 018 bp was cloned using RT-PCR. Then,plant expression vectors with sense and antisense orientations and RNA interfering was constructed and transferred into Agrobacterium tumefaciens LBA4404 by electoral transformation methods . |
| |
| Keywords: | cDNA Tomato(Lycopersicon esculentum) ACO gene cDNA Construction of expression vectors |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
