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| 氮素对NaCl胁迫下甜高粱种子萌发及芽苗生长与生理的影响 | |
| 引用本文： | 穆 静,刘小京,徐 进,毛任钊,魏 巍,杨莉琳.氮素对NaCl胁迫下甜高粱种子萌发及芽苗生长与生理的影响[J].中国生态农业学报,2012,20(10):1303-1309. |
| 作者姓名： | 穆 静 刘小京 徐 进 毛任钊 魏 巍 杨莉琳 |
| 作者单位： | 1. 中国科学院农业水资源重点实验室中国科学院遗传与发育生物学研究所农业资源研究中心 石家庄050022;中国科学院研究生院 北京 100049 2. 中国科学院农业水资源重点实验室中国科学院遗传与发育生物学研究所农业资源研究中心 石家庄050022 3. 中国农业大学教学实验农场 涿州 072750 |
| 基金项目： | 国家科技支撑计划项目(2009BADA7B05)和公益性行业(农业)科研专项经费项目(200903001-7)资助 |
| 摘 要： | 为了研究NaCl胁迫下氮肥对甜高粱种子萌发及芽苗生长和生理特性的影响,探索提高甜高粱耐盐能力的措施,室内设置不同盐分浓度、不同氮源及浓度条件下甜高粱萌芽试验。结果表明:NaCl胁迫和不同氮源对甜高粱发芽和芽苗生长的影响各有不同。NaCl浓度对甜高粱种子萌发有显著影响,在甜高粱芽苗生长阶段,通过提高保护酶活性和渗透调节物质而增强耐盐伤害能力是有限的。100 mmol.L 1NaCl胁迫下,根系POD活性最低,而叶片MDA积累量、可溶性糖含量、POD活性最高,受盐害程度最大。没有盐胁迫情况下增加不同氮源及氮量对甜高粱根叶生理特性的影响差异显著,当氮浓度在20 mmol.L 1时,细胞受伤害程度最低,生长最好。不同形态氮源对甜高粱发芽和幼苗生长的影响差异明显,NH4Cl的促进效果优于KNO3。在100mmol.L 1的NaCl胁迫下,施加铵态氮或硝态氮源均可以增强甜高粱芽苗期的POD活性,减少MDA积累,从而缓解盐胁迫带来的伤害。研究表明采取适当的氮肥调控措施可以提高甜高粱的耐盐能力。 |
| 关 键 词： | 甜高粱 盐胁迫 氮源 发芽 幼苗生长 生理特性 |
| 收稿时间： | 5/2/2012 12:00:00 AM |
| 修稿时间： | 7/9/2012 12:00:00 AM |
Effects of nitrogen on sweet sorghum seed germination, seedling growth and physiological traits under NaCl stress |
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| MU Jing,LIU Xiao-Jing,XU Jin,MAO Ren-Zhao,WEI Wei and YANG Li-Lin.Effects of nitrogen on sweet sorghum seed germination, seedling growth and physiological traits under NaCl stress[J].Chinese Journal of Eco-Agriculture,2012,20(10):1303-1309. | |
| Authors: | MU Jing LIU Xiao-Jing XU Jin MAO Ren-Zhao WEI Wei and YANG Li-Lin |
| Institution: | 1. Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China;Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China;Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China;Teaching & Experimental Farm of China Agricultural University, Zhuozhou 072750, China;Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China |
| Abstract: | The effects of nitrogen application on germination, seedling growth and physiological traits of sweet sorghum Sorghum bicolor (L.) Moench] were investigated under NaCl stress. The aim of the study was to enhance salt-tolerance of sweet sorghum through laboratory germination experiments at different salt and nitrogen (N) concentrations using two N sources. The results showed that either salt or N significantly influenced seed germination and seedling growth of sweet sorghum. Under NaCl stress, sweet sorghum salt uptake increased via improved enzyme activities and osmoregulation during seed germination and bud seedling growth stages. Under NaCl stress, there was a little ability for sweet sorghum to resist salt by improving enzyme activity and osmoregulation substance during seed germination and seedling growth stage. Lowest root POD activity, highest leaf MDA accumulation, leaf soluble sugar and leaf POD under 100 mmol·L-1 NaCl stress indicated the greatest salt damage to sweet sorghum. N treatments without NaCl markedly affected seed germination and seedling growth of sweet sorghum. Sweet sorghum seedling grew best under 20 mmol(N)·L-1 due to lessening cell damage by NaCl. NH4Cl improved more than KNO3 in seed germination and seedling growth of sweet sorghum. Under 100 mmol·L-1 NaCl stress, supplementation with either form of the two N sources (NH4+ and NO3- ) ameliorated adverse salt stress effects on seedling growth and examined physiological traits. This was possible by improving POD activity and reducing MDA accumulation. It was evident that an appropriate nitrogen fertilizer application improved the salt tolerability of sweet sorghum. |
| Keywords: | Sweet sorghum Salt stress Nitrogen source Seed germination Seedling growth Physiological trait |
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