| 半夏试管块茎直接再生技术的研究 |
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| 引用本文： | 薛建平,朱艳芳,张爱民,柳俊.半夏试管块茎直接再生技术的研究[J].作物学报,2004,30(10):1060-1064. |
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| 作者姓名： | 薛建平 朱艳芳 张爱民 柳俊 |
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| 作者单位： | 淮北煤炭师范学院生物系, 安徽淮北235000 |
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| 摘 要： | 选用半夏的叶片、叶柄为试验材料，设计不同的培养基，研究植物生长物质种类和浓度、糖种类和浓度、叶柄的放置情况及光照与黑暗等条件对直接再生形成试管小块茎的影响。结果表明,叶片直接诱导块茎的适宜培养基为MS附加0.5 mg/L 6-BA、0.5 mg/L IAA 和3％蔗糖；叶柄直接诱导块茎的适宜培养基为MS附加0.5 mg/L 6-BA、0.5 mg/L
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| 关 键 词： | 半夏 叶片 叶柄 试管块茎 组织培养 |
| 收稿时间： | 2003-06-04 |
Research on Direct Formation of Microtubers from Pinellia ternata |
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| XUE Jian-Ping ,ZHU Yan-Fang,ZHANG Ai-Min,LIU Jun.Research on Direct Formation of Microtubers from Pinellia ternata[J].Acta Agronomica Sinica,2004,30(10):1060-1064. |
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| Authors: | XUE Jian-Ping ZHU Yan-Fang ZHANG Ai-Min LIU Jun |
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| Institution: | Department of Biology, Huaibei Coal Industry Teachers’ College，Huaibei 235000, Anhui |
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| Abstract: | Under the bright or dark condition, leaves and petioles of Pinellia ternata with different inoculation position were cultured on media with different plant growth substances and sugars. The results showed that the optimum medium to direct formation of microtubers from leaves was MS medium with 0.5 m |
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| Keywords: | Pinellia ternata Leaf Petiole Microtubers Tissue culture |
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