| 乙型肝炎病毒X基因真核表达载体的构建 |
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| 引用本文： | 卢宏柱,徐友伦.乙型肝炎病毒X基因真核表达载体的构建[J].长江大学学报,2009,6(1). |
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| 作者姓名： | 卢宏柱 徐友伦 |
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| 作者单位： | [1]长江大学临床医学院长江大学附属第一医院,湖北荆州434000 [2]长江大学工程技术学院,湖北荆州434023 |
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| 摘 要： | 目的：构建HBVX基因与pCI-neo相融合的高效真核表达载体。方法：设计并合成HBVX基因的引物，以HBVDNA阳性血清PCR扩增得到HBVX基因全序列，将X基因连接到真核表达载体pCI—neo上后，酶切图谱分析、PCR检测和扩增产物序列分析等，鉴定所构建的真核表达载体。结果：以重组载体为模板扩增得到的片段大小与已知HBVX基因大小相同，酶切也得到目的基因片段，测序结果也显示与已知X基因序列相同。结论：成功构建了HBVX基因的真核表达载体，为进一步研究HBVX基因及其产物的功能奠定了基础。
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| 关 键 词： | 乙型肝炎病毒 X基因 真核表达载体 |
Construction of Hepatitis B Virus X Gene Eukaryotic Expression Vector |
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| LU Hong-zhu XU You-lun.Construction of Hepatitis B Virus X Gene Eukaryotic Expression Vector[J].Journal of Yangtze University,2009,6(1). |
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| Authors: | LU Hong-zhu XU You-lun |
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| Institution: | Clinic Medical School of Yangtze University;Jingzhou 434000;China;Engineering Institute of Technology of Yangtze University;Jingzhou 434023;China |
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| Abstract: | Objective:To construct a highly effective eukaryotic expression vector pCI-neo with HBV X gene(pCI-neo-X).Methods:HBV DNA was extracted from the HBV DNA positive serum.HBV X gene was amplified by PCR,and identified by gelose electrophoresis analysis.The PCR products and the eukaryotic expression vectors pCI-neo were digested by restriction endonucleases,then the digested X gene was inserted into the digested eukaryotic expression vector.After screening,the positive recombinants were picked out.Results:PCR a... |
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| Keywords: | Hepatitis B virus X gene Eukaryotic expression vector |
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