| 马传贫驴白细胞弱毒疫苗株基质蛋白基因的克隆与表达 |
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| 引用本文： | 薛飞,朱远茂,王晓钧,杨建德,相文华,刘志英,赵立平,吕晓玲,沈荣显.马传贫驴白细胞弱毒疫苗株基质蛋白基因的克隆与表达[J].中国预防兽医学报,2003,25(1):1-4. |
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| 作者姓名： | 薛飞 朱远茂 王晓钧 杨建德 相文华 刘志英 赵立平 吕晓玲 沈荣显 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所疫病室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码基质蛋白（p15)的基因，并在大肠杆菌中进行了表达，所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化，在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应，这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制及在接种马体人免疫应答的研究中。
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| 关 键 词： | 基质蛋白 马 传染性贫血病 马传贫驴白细胞弱毒疫苗株 基因克隆 基因表达 p15基因 |
| 文章编号： | 1008-0589(2003)01-0001-04 |
| 修稿时间： | 2002年6月21日 |
Cloning and Expression of Matrix Protein(p15) from Donkey Leukocyte Attenuated Vaccine Strain of Equine Infectious Anemia Virus |
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| XUE Fei,ZHU Yuan-mao,WANG Xiao-jun,YANG Jian-de,XIANG Wen-hua,LIU Zhi-ying,ZHAO Li-ping,LU Xiao-ling,SHEN Rong-xian.Cloning and Expression of Matrix Protein(p15) from Donkey Leukocyte Attenuated Vaccine Strain of Equine Infectious Anemia Virus[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(1):1-4. |
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| Authors: | XUE Fei ZHU Yuan-mao WANG Xiao-jun YANG Jian-de XIANG Wen-hua LIU Zhi-ying ZHAO Li-ping LU Xiao-ling SHEN Rong-xian |
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| Abstract: | Gene coding for matrix protein (p15) of donkey leukocyte attenuated vaccine strain of equine infectious anemia virus(EIAV) was cloned from its proviral DNA obtained from the vaccine strain infected donkey leukocyte cultures,and expressed in E.coli. Protein was expressed in a soluble form as fusion protein.It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.Recombinant matrix protein showed reactivity to EIAV positive serum samples and no reactivity to normal horse sera in indirect ELISA and immunoblot assay.This demonstrates that recombinant p15 protein has very good antigenicity and specificity and may be used for research on the in vitro and in vivo replication and immune responses in the vaccinated horses of donkey leukocyted attenuated vaccine strain of equine infectious anemia virus. |
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| Keywords: | Equine infectious anemia Recombinant p15 matrix protein Donkey leukocy teadapted attenuated vaccine strain of equine infectious anemia virus |
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