| Patatin、Wun1启动子驱动的PPO反义基因植物表达载体的构建 |
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| 引用本文： | 陈春艳,王清.Patatin、Wun1启动子驱动的PPO反义基因植物表达载体的构建[J].中国马铃薯,2006,20(3):140-144. |
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| 作者姓名： | 陈春艳 王清 |
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| 作者单位： | 1. 甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070;甘肃农业大学农学院,甘肃,兰州,730070
2. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070 |
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| 摘 要： | 以植物表达载体PC3-ASPPOty为基础,分别构建了由Patatin、Wun1启动子调控的PPO反义基因植物表达载体pCP-ASPPOty、pCW-ASPPOty,并通过直接导入法将重组质粒分别导入根癌农杆菌LBA4404中,经PCR鉴定证明,此基因已整合到农杆菌Ti上。表达载体的建立为获得地上部分PPO活性正常、块茎损伤低褐化的马铃薯加工型改良品种奠定了基础。
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| 关 键 词： | Patatin启动子 Wun1启动子 PPO反义基因 表达载体 |
| 文章编号： | 1672-3635（2006）03-0140-05 |
| 收稿时间： | 2005-12-29 |
| 修稿时间： | 2005年12月29 |
Construction of Antisense PPO Gene Plant Expression Vector Controlled by Patatin or Wound-inducible Promoter |
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| Chen Chunyan,Wang Qing.Construction of Antisense PPO Gene Plant Expression Vector Controlled by Patatin or Wound-inducible Promoter[J].Chinese Potato,2006,20(3):140-144. |
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| Authors: | Chen Chunyan Wang Qing |
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| Institution: | 1. Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China; 2. College of Agronomy,Gansu Agriculture University, Lanzhou, Gansu 730070, China; 3. College of Life Science and Technology, Gansu Agriculture University, Lanzhou, Gansu 730070, China |
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| Abstract: | Two plant expression vectors were constructed in this experiment. Plasmid pCP-ASPPOty and pCW-ASP.POty were derived from pC3-ASPPOty, with PPO antisense gene being controlled by Patatin promoter and Wound-inducible promoter, respectively. Then, the recombination plasmids were introduced into Agrobacteriurn LBA4404 by direct entering ways. It was proven that the vectors were integrated into LBA4404. |
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| Keywords: | patatin promoter wound-inducible promoter PPO antisense gene expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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