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饲料中维生素C对大菱鲆繁殖性能的影响
作者姓名:张海涛  梁萌青  郑珂珂  王新星
作者单位:中国海洋大学,青岛 266100;中国水产科学研究院黄海水产研究所,青岛 266071;中国水产科学研究院黄海水产研究所,青岛 266071;中国水产科学研究院黄海水产研究所,青岛 266071;中国水产科学研究院黄海水产研究所,青岛 266071
基金项目:鲆鲽类产业技术体系科研专项经费(nycytx-50-G07)
摘    要:在基础饲料中分别添加0、800和4 800mg/kg的维生素C饲养大菱鲆亲鱼240d,通过比较各处理组亲鱼的生长、性腺指数、相对产卵量、上浮卵率、孵化率、卵径以及各组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究维生素C对大菱鲆亲鱼繁殖性能的影响。实验结果表明,各处理组亲鱼生长无显著差异(P>0.05),但随着饲料中维生素C添加量的增加,亲鱼的生长呈上升趋势。800和4 800mg/kg组亲鱼相对产卵量显著高于对照组(P<0.05)。随着饲料中维生素C添加量的增加,亲鱼性腺指数、产卵次数、上浮卵率、受精率和孵化率均显著提高(P<0.05),亲鱼卵和组织中维生素C含量反映了饲料中的维生素C含量。虽然各处理组亲鱼的卵径和油球径差异均不显著(P>0.05),但随着饲料中维生素C添加量的增加,亲鱼卵径和油球径呈上升的趋势。肝脏、卵巢和血清中SOD活性随饲料中维生素C添加量的增加而显著升高(P<0.05),而MDA含量则显著下降(P<0.05)。综上所述,大菱鲆亲鱼饲料中添加维生素C,能有效改善大菱鲆的繁殖性能。

关 键 词:大菱鲆  亲鱼  饲料  维生素C  繁殖性能
收稿时间:2012/3/11 0:00:00
修稿时间:2012/4/12 0:00:00

Impact of dietary vitamin C on the reproductive performance of turbot Scophthalmus maximus
Abstract:An eight-month feeding experiment was conducted to determine the impact of dietary vitamin C on the reproductive performance of turbot Scophthalmus maximus.The basal diet was supplemented with 0,800 and 4 800 mg/kg vitamin C to formulate three experimental diets.Results showed that the growth of turbot broodstock was improved with the increasing dietary vitamin C, but no significant difference was observed between control and vitamin C supplementation treatments.The egg production of female broodstock fed vitamin C supplemented diets was significantly (P<0.05) higher than those fed the basal diet.The gonadosomatic index (GSI),number of spawns,percentage of buoyant egg,fertilization rate and hatching rate of 800 mg/kg and 4 800 mg/kg treatments were significantly higher with the increase of vitamin C addition in diets(P<0.05). Vitamin C concentrations in eggs reflected dietary vitamin C addition.The egg diameter and oil globule diameter of turbot broodstock was improved with the increasing dietary vitamin C,but no significant difference was observed between control and vitamin C supplementation treatments.With the increase of dietary vitamin C, the activities of superoxide ismutase (SOD) in tissues were significantly reduced (P<0.05),but malonaldehyde (MDA) contents were significantly increased (P<0.05).So,it is concluded that vitamin C supplement in the diets can improve the reproductive performance of turbot broodstock.
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