| 铁核桃叶片多酚类物质含量及其抗氧化活性 |
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| 引用本文： | 史斌斌,张文娥,李 雪,潘学军.铁核桃叶片多酚类物质含量及其抗氧化活性[J].园艺学报,2017,44(1):23-32. |
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| 作者姓名： | 史斌斌 张文娥 李 雪 潘学军 |
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| 作者单位： | 贵州大学，贵州省果树工程技术研究中心，贵阳 550025 |
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| 基金项目： | 国家科技支撑计划课题（2014BAD23B03）；贵州省高层次创新型人才培养项目[黔科合人才（2016）4038号] |
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| 摘 要： | 以铁核桃(Juglans sigillata)‘黔核7号’叶片为试材,研究了叶片发育过程中多酚类物质含量及其体外抗氧化活性的变化。结果表明:铁核桃叶片多酚类物质含量在其整个生长周期呈双峰曲线变化规律,4月12日至5月11日上升,5月11日至7月6日下降,7月6日至8月1日大幅上升,8月1日至9月19日逐渐下降。HPLC鉴定出没食子酸、儿茶素、绿原酸、咖啡酸、丁香酸、丁香醛、对香豆酸、阿魏酸、芦丁、槲皮素、杨梅素、胡桃醌,共12种单体酚;含量最高的为芦丁,其次为绿原酸;含量较低的为对香豆酸、没食子酸。5月是铁核桃叶片中大多数单体酚含量降低的时期,而8月是部分单体酚含量升高的时期。总酚、总黄酮含量与还原能力和ABTS自由基清除能力相关性较高,单体酚与抗氧化活性相关性较低。铁核桃叶片多酚类物质含量丰富,抗氧化活性强,其动态变化与叶片发育阶段及外界环境条件变化密切相关。
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| 关 键 词： | 核桃 叶片 多酚 单体酚 抗氧化活性 相关性 |
Phenolics Content and Antioxidant Activity of Walnut（Juglans sigillata）Leaves |
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| SHI Binbin,ZHANG Wene,LI Xue,PAN Xuejun.Phenolics Content and Antioxidant Activity of Walnut（Juglans sigillata）Leaves[J].Acta Horticulturae Sinica,2017,44(1):23-32. |
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| Authors: | SHI Binbin ZHANG Wene LI Xue PAN Xuejun |
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| Institution: | Guizhou Engineering Research Center for Fruit Crops，Guizhou University，Guiyang 550025，China |
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| Abstract: | In this study，phenolics content and antioxidant activity of （Juglans sigillata Dode.‘Qianhe 7’）leaves in vitro were analysis at 9 developmental stages. The results showed that phenolics content presented a trend of bimodal curve during the annual growing period. It increased from April 12 to May 11，then decreased until July 6 and increased rapidly again until August 1. Subsequently，phenolics content gradually reduced until September 19. Twelve kinds of monophenols，including gallic acid，catechin，chlorogenic acid，caffeic acid，syringic acid，syringaldehyde，p-coumaric acid，ferulic acid，rutin，myricetin，quercetin and juglone，were identified by HPLC method，in which rutin was observed at the highest content，followed by chlorogenic acid. Contents of p-coumaric acid and gallic acid were in lower levels，respectively. Most of monophenols decreased in May，but part of them increased in August. Both total phenolics and total flavones contents highly correlated with antioxidant activity as determined by FRAP or ABTS methods，respectively，while lower correlations between monophenols and antioxidant activities was observed in this study. Walnut leaves are rich in ，which lead to strong antioxidant activity. Phenolics content and antioxidant activity changed regularly with development of walnut leaves and variation of environment.phenolics contentwalnut |
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| Keywords: | walnut Juglans sigillata Juglans sigillata leaf phenolics monophenols antioxidant activity correlation |
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