| 结球甘蓝CML家族基因及其在授粉后柱头中的表达 |
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| 引用本文： | 蒲 敏,,罗绍兰,廉小平,曾 静,张贺翠,刘倩莹,左同鸿,朱利泉.结球甘蓝CML家族基因及其在授粉后柱头中的表达[J].园艺学报,2018,45(11):2129-2140. |
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| 作者姓名： | 蒲 敏 罗绍兰 廉小平 曾 静 张贺翠 刘倩莹 左同鸿 朱利泉 |
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| 作者单位： | （1西南大学农学与生物科技学院，重庆 400715；2重庆市蔬菜重点实验室，重庆 400715；3长江师范学院生命科学与技术学院，重庆 408100；4涪陵第十四中学校，重庆 408000） |
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| 摘 要： | 利用NCBI和甘蓝基因组数据库筛选获得了76个结球甘蓝CML家族基因，其随机分布在甘蓝的9条染色体上，染色体上最多的有12个基因，最少有1个基因。分析发现该基因家族编码蛋白氨基酸残基长度在109 ~ 365个之间，分子量大多在20 kD左右，为小分子蛋白，且均含有2 ~ 4个保守的EF-hand结构域。甘蓝的76个CML基因依据与拟南芥的CML家族的进化关系及序列相似性分为Ⅰ~ Ⅷ个亚家族，每个亚家族基因有较高的同源性，进化关系较近。经亚细胞预测分析发现大部分蛋白在质膜和胞外表达，少量在质膜、细胞核内和叶绿体等部位表达。数字表达谱分析发现在76个基因中，有25个基因在自花和异花授粉后差异表达，其中BoCML12、BoCML42、BoCML44、BoCML45、BoCML60、BoCML70、BoCML71 和BoCML74等8个基因在自花和异花授粉后表达趋势有较大差异，表明这8个基因以不同的方式参与调控甘蓝自花和异花授粉后的反应过程，有可能与甘蓝的自交不亲和性相关。
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| 关 键 词： | 甘蓝 CML家族基因 生物信息学 表达分析 |
Bioinformatics and Expression Analysis After Pollination of Stigma of CML Family Genes in Brassica oleracea var. capitata |
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| PU Min,,LUO Shaolan,LIAN Xiaoping,ZENG Jing,ZHANG Hecui,LIU Qianying,ZUO Tonghong,ZHU Liquan,.Bioinformatics and Expression Analysis After Pollination of Stigma of CML Family Genes in Brassica oleracea var. capitata[J].Acta Horticulturae Sinica,2018,45(11):2129-2140. |
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| Authors: | PU Min LUO Shaolan LIAN Xiaoping ZENG Jing ZHANG Hecui LIU Qianying ZUO Tonghong ZHU Liquan |
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| Institution: | （1College of Agronomy and Biotechnology，Southwest University，Chongqing 400715，China；2Key Laboratory in Olericulture of Chongqing，Chongqing 400715，China；3School of Life Science and Biotechnology，Yangtze Normal University，Chongqing 408100，China；4Fuling No. 14 Middle School，Chongqing 408000，China） |
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| Abstract: | Seventy-six CML family genes in Brassica oleracea var. capitata were identified and characterized through B.oleracea genomics database and NCBI database.They are randomly distributed on 9 chromosomes. There are 12 genes at the most，and at least 1 gene. It was found that the amino acid residues of the family of encoded proteins ranged from 109 to 365，and the molecular weight was mostly about 20 kD. They were small molecular proteins and contained 2–4 conserved EF-hand domains. Phylogenetic analysis showed that these CML family genes could be classified into eight groups，
combining with Arabidopsis CMLs and each subfamily genes have high homology and close evolutionary relationship. Subcellular predictive analysis showed that most of the proteins were expressed in the plasma membrane and extracellular membrane，and a few in the plasma membrane，nucleus and chloroplast. Among the 76 genes，25 genes were differentially expressed after self-pollination and cross-pollination. Among them，8 genes，such as BoCML12，BoCML42，BoCML44，BoCML45，BoCML60，BoCML70，BoCML71and BoCML74，have significant expression differences. The results indicated that these 8 genes were involved in the regulation of self-pollination and cross-pollination of cabbage in different ways and may be related to the self-incompatibility of cabbage. |
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| Keywords: | Brassica oleracea var capitata CML family genes bioinformatics expression analysis |
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