| 基于SSR标记的板栗地方品种遗传多样性与关联分析 |
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| 引用本文： | 江锡兵,汤 丹,龚榜初,赖俊声.基于SSR标记的板栗地方品种遗传多样性与关联分析[J].园艺学报,2015,42(12):2478-2488. |
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| 作者姓名： | 江锡兵 汤 丹 龚榜初 赖俊声 |
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| 作者单位： | 1中国林业科学研究院亚热带林业研究所，浙江省林木育种技术研究重点实验室，杭州 311400；2庆元县林业局，浙江庆元 323800 |
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| 基金项目： | 浙江省竹木农业新品种选育重大科技专项重点项目（2012C12908-18）；中国林业科学院亚热带林业研究所基本科研业务费
专项资金项目（RISF61253） |
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| 摘 要： | 采用SSR标记对山东等10个省份95个板栗地方品种的遗传多样性、群体结构进行分析,并进行板栗18个农艺性状与SSR标记关联分析。结果表明:(1)17对SSR引物在95个板栗品种中检测出44个等位位点,平均为2.6个,Shannon’s指数(I)和多态性信息含量(PIC)平均值分别为0.67和0.352,遗传多样性较为丰富;(2)山东群体和江苏群体的多态性位点比率(P)最高,均达到94.12%,观察等位基因数(Na)分别为2.53和2.18,亦高于其他群体;(3)根据Evanno等统计模型,92个板栗品种被划分为3个亚群,分别包含25、40和27个品种,且均有着复杂的地理起源,另有3个品种没有明确的类群归属;(4)利用GLM和MLM模型进行关联分析,并经过假阳性检验,发现叶柄长度和淀粉含量分别与标记Cs CAT 5和Cs CAT 22显著关联,关联系数分别为0.4027和0.1869。
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| 关 键 词： | 板栗 SSR 遗传多样性 群体结构 关联分析 |
Genetic Diversity and Association Analysis of Local Cultivars of Chinese Chestnut Based on SSR Markers |
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| JIANG Xi-bing,TANG Dan,GONG Bang-chu,LAI Jun-sheng.Genetic Diversity and Association Analysis of Local Cultivars of Chinese Chestnut Based on SSR Markers[J].Acta Horticulturae Sinica,2015,42(12):2478-2488. |
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| Authors: | JIANG Xi-bing TANG Dan GONG Bang-chu LAI Jun-sheng |
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| Institution: | 1Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Key Laboratory of Tree Breeding of Zhejiang Province，Hangzhou 311400，China；2Forestry Administration of Qingyuan County，Qingyuan，Zhejiang 323800，China |
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| Abstract: | Genetic diversity and population structure of 95 local cultivars of Chinese chestnut （Castanea mollissima BL.）from 10 provinces，such as Shandong etc.，were analyzed using SSR markers，and association analysis was conducted with 18 agronomic traits，all of these laid the theoretical basis for rational use and protection of the germplasm resources，and molecular marker-assisted breeding of Chinese chestnut. The results showed：（1）Forty-four allelic loci were detected from 95 chestnut cultivars in 17 SSR markers，the average was 2.6，and the mean of Shannon’s index（I）and polymorphism information content （PIC）were 0.67 and 0.352，respectively，which reveal that the genetic diversity were abundant；（2）Percentage of polymorphic loci of Shandong and Jiangsu population reach the highest of 94.12%，and observed number of alleles（Na）of them were 2.53 and 2.18，respectively，which also higher than the other provinces，indicated that genetic diversity of them were higher than the other provinces；（3）Ninety-two chestnut cultivars were divided into three subgroups based on statistical model of Evanno etc.，the three subgroups contain 25，40 and 27 cultivars，respectively，and their geographical origin were highly complexly，however，the other three cultivars were clearly not assigned to any subgroup；（4）Petiole length and starch cotent were significantly associated with CsCAT5 and CsCAT22 markers after a false positive test by using GLM and MLM models，and their association coefficient were 0.4027 and 0.1869，repectively. |
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| Keywords: | Chinese chestnut SSR genetic diversity population structure association analysis |
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