| 平邑甜茶MhSnRK1在番茄中超表达对植株碳代谢的影响 |
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| 引用本文： | 王贵芳,彭福田,张亚飞,党祝庆,王娜娜.平邑甜茶MhSnRK1在番茄中超表达对植株碳代谢的影响[J].园艺学报,2014,41(11):2188-2195. |
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| 作者姓名： | 王贵芳 彭福田 张亚飞 党祝庆 王娜娜 |
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| 作者单位： | 山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，山东泰安 271018 |
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| 基金项目： | 国家现代农业产业技术体系建设专项资金项目（CARS-31-3-03）；山东省高校优秀科研创新团队计划项目 |
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| 摘 要： | 以超表达平邑甜茶（Malus hupehensis Rehd. var. pingyiensis Jiang）SnRK1（蔗糖非发酵–1–型相关蛋白激酶–1）基因MhSnRK1的番茄株系O-7及野生型番茄（Solanum lycopersicum）为试材，研究MhSnRK1对番茄植株碳代谢的影响。结果表明，与野生型番茄相比，超表达MhSnRK1番茄数字基因表达谱分析显示，光合途径中差异表达的10个基因中7个基因转录被上调；功能叶片的净光合速率日变化平均值提高20.3%；嫩叶及功能叶SnRK1酶活性比野生型功能叶提高20.6%和25.0%，可溶性糖提高57.7%和27.0%；超表达MhSnRK1番茄嫩叶SnRK1酶活性明显高于功能叶，淀粉含量提高了近1倍，而野生型番茄嫩叶及功能叶差异不明显。海藻糖处理后，超表达MhSnRK1番茄和野生型番茄净光合速率都下降，且野生型下降幅度大于超表达MhSnRK1番茄；超表达MhSnRK1及野生型番茄嫩叶和功能叶SnRK1酶活性都降低，但超表达MhSnRK1番茄仍高于野生型。
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| 关 键 词： | 平邑甜茶 SnRK1 番茄 数字基因表达谱 可溶性糖 淀粉 净光合速率 |
| 收稿时间： | 2013-05-15 |
Effects of Overexpressing Pingyi Tiancha MhSnRK1 on Carbohydrate Metabolism in Tomato |
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| WANG Gui-Fang,PENG Fu-Tian-,ZHANG Ya-Fei,DANG Zhu-Qing,WANG Na-Na.Effects of Overexpressing Pingyi Tiancha MhSnRK1 on Carbohydrate Metabolism in Tomato[J].Acta Horticulturae Sinica,2014,41(11):2188-2195. |
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| Authors: | WANG Gui-Fang PENG Fu-Tian- ZHANG Ya-Fei DANG Zhu-Qing WANG Na-Na |
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| Institution: | College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China |
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| Abstract: | MhSnRK1-overexpressing tomato（O-7）and wild type tomato were used to investigate the function of MhSnRK1 on carbohydrate metabolism. The results showed that ten genes in photosynthesis pathway were differentially expressed between MhSnRK1-overexpressing line and wild type，among which seven genes were up-regulated in MhSnRK1-overexpressing line through digital gene expression（DGE）analysis. Compared with wild type tomato，the average photosynthetic rates increased by 20.3% in mature leaves of MhSnRK1-overexpressing tomato. SnRK1 activities in young and mature leaves increased respectively by 20.6% and 25.0% of MhSnRK1-overexpressing tomato. The soluble sugar contents in young leaves and in mature leaves increased by 57.7% and 27.0% respectively. In young leaves of MhSnRK1-overexpressing tomato SnRK1 activity was obviously higher than that in mature leaves. Thestarch contents were nearly doubled in young leaves of MhSnRK1-overexpressing tomato，while there was no obvious difference in starch content between these leaves of the wild type. After trehalose treatments，the photosynthetic rates decreased in mature leaves of MhSnRK1-overexpressing and wild type tomato and wild type tomato decreased more. SnRK1 activities in young and mature leaves of MhSnRK1-over- expressing and wild type tomato both decreased，while MhSnRK1-overexpressing line was still higher than wild type tomato. |
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| Keywords: | Malus hupehensis Rehd var pingyiensis Jiang SnRK1 tomato digital gene expression-tag profiling soluble sugar starch photosynthetic rate |
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