| 苹果蔗糖转运蛋白MdSUT2调控花青苷积累的研究 |
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| 引用本文： | 路 静,马齐军,刘 晓,康 慧,刘亚静,郝玉金,由春香.苹果蔗糖转运蛋白MdSUT2调控花青苷积累的研究[J].园艺学报,2019,46(1):1-7. |
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| 作者姓名： | 路 静 马齐军 刘 晓 康 慧 刘亚静 郝玉金 由春香 |
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| 作者单位： | （山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，农业部黄淮地区园艺作物生物学与种质创制重点实验室，山东泰安 271018） |
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| 摘 要： | 从‘嘎拉’苹果(Malus×domestica Borkh.)中克隆蔗糖转运蛋白基因MdSUT2(序列号:MDP0000277235),该基因包含1 704 bp开放阅读框,编码566个氨基酸,蛋白大小为56 kD。构建MdSUT2表达载体并利用农杆菌介导法侵染拟南芥,半定量RT-PCR证明Md SUT2在拟南芥中异源表达。构建瞬时表达载体,‘红星’苹果进行Md SUT2瞬时表达,其花青苷含量增多,而沉默该基因花青苷含量明显降低。
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| 关 键 词： | 苹果 MdSUT2 基因克隆 功能鉴定 花青苷 |
Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2 |
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| LU Jing,MA Qijun,LIU Xiao,KANG Hui,LIU Yajing,HAO Yujin,YOU Chunxiang.Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2[J].Acta Horticulturae Sinica,2019,46(1):1-7. |
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| Authors: | LU Jing MA Qijun LIU Xiao KANG Hui LIU Yajing HAO Yujin YOU Chunxiang |
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| Institution: | Department of Pathology, Air Force Medical University, Xi'an 710032, China |
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| Abstract: | A gene named MdSUT2（MDP0000277235）was cloned from‘Gala’apple（Malus × domestica Borkh.）. Sequence analysis showed that this gene contained 1 704 bp nucleotides，and encoded a protein of 566 amino acids. Overexpression vector of MdSUT2 was constructed and transformed into Arabidopsis seedlings via Agrobacterium-mediated method. Semi RT-PCR analysis indicated that MdSUT2 was genetically transformed into Arabidopsis. In addition，the transient expression vectors were constructed and transformed into apple fruits. We found that overexpression of MdSUT2 in apple fruits significantly increased the anthocyanin content，whereas suppression of MdSUT2 decreased the anthocyanin content. |
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| Keywords: | apple MdSUT2 gene clone functional identification anthocyanin |
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