| 20个苹果品种的S基因型鉴定 |
| |
| 引用本文： | 丁体玉,吴萌萌,张瑞萍,阎振立,闫亚茹,陈迪新,张恒涛,高启明.20个苹果品种的S基因型鉴定[J].园艺学报,2018,45(12):2277-2282. |
| |
| 作者姓名： | 丁体玉 吴萌萌 张瑞萍 阎振立 闫亚茹 陈迪新 张恒涛 高启明 |
| |
| 作者单位： | 1中国农业科学院郑州果树研究所，郑州 450009；2河南科技大学，河南洛阳 471003 |
| |
| 基金项目： | 国家自然科学青年基金项目（31401835）；国家现代农业产业技术体系建设专项资金项目（CARS-28）；中国农业科学院科技创新工程项目（CAAS-ASTIP-2016-RIP-02） |
| |
| 摘 要： | 以‘富士’、‘华瑞’、‘华硕’和‘华星’等20个苹果品种为材料,利用S等位基因高度保守氨基酸序列FTQQYQ和anti-1/MIWPNV设计的S基因通用引物,以及S等位基因多态性序列设计的19对特异引物,PCR扩增、测序以鉴定20个品种的S基因型;并用‘华瑞’和‘华硕’分别与‘美八’、‘锦秀红’、‘华冠’和‘富士’进行授粉试验验证S基因型的准确性。PCR结果表明:通用引物扩增S等位基因时,仅‘富士’、‘华瑞’、‘华硕’、‘华星’、‘美八’和‘红脆宝’6个品种有效地扩增出2条特异的S等位基因条带,其S基因型有S1S_9、S_9S_(24)、S_5S_9和S_5S_(24)等4种;19对特异引物扩增S等位基因时,‘华帅’等14个品种扩增得到2条特异性条带,S基因型有S_(10)S_(19)、s_2s_3、s_2S_5、s_3S_(10)、s_2S_9、S_5S_(24)、S_9S_(10)、s_3S_(10)和S_5S_9等9种。因此,20个苹果品种的S基因型分别为:‘富士’S1S_9,‘华瑞’和‘华硕’S_9S_(24),‘华星’、‘美八’和‘红珍珠’S_5S_9,‘红脆宝’、‘华玉’和‘99-1-29’S_5S_(24),‘华帅’S_(10)S_(19),‘金玉’s_2s_3,‘早红’、‘华美’和‘嘎拉’s_2S_5,‘Seokwang’s_3S_(10),‘锦秀红’、‘蜜玉’和‘华冠’s_2S_9,‘绿佳’S_9S_(10),‘信浓红’s_3S_(10)。2015和2016年‘华瑞’与‘华硕’的正反交组合坐果率较低(低于15.52%);而‘华瑞’和‘华硕’分别与‘美八’、‘锦秀红’和‘华冠’、‘富士’品种的正反交组合坐果率较高(高于46.30%)。因此,本试验中相同S基因型的授粉组合其坐果率较低,不同S基因型的授粉组合其坐果率较高,授粉试验支持S基因型鉴定结果。
|
| 关 键 词： | 苹果 自交不亲和性 S基因型 |
Identification of S-genotypes of 20 Apple Cultivars |
| |
| DING Tiyu,WU Mengmeng,ZHANG Ruiping,YAN Zhenli,YAN Yaru,CHEN Dixin,ZHANG Hengtao,GAO Qiming.Identification of S-genotypes of 20 Apple Cultivars[J].Acta Horticulturae Sinica,2018,45(12):2277-2282. |
| |
| Authors: | DING Tiyu WU Mengmeng ZHANG Ruiping YAN Zhenli YAN Yaru CHEN Dixin ZHANG Hengtao GAO Qiming |
| |
| Institution: | 1. Department of Laboratory Animal, Guangzhou Medical University, Guangzhou 511436, China;
2. Department of Chinese and Western Medicine, Guangzhou Medical University, Guangzhou 511436, China;
3. College of Pharmacy, Guangzhou Medical University, Guangzhou 511436, China |
| |
| Abstract: | To identify 20 apple germplasms’ S-genotypes by PCR and sequencing，the universal primers and 19 pairs of specific primers of apple S-alleles were designed for‘Fuji’，‘Huarui’，‘Huashuo’，‘Huaxing’and so on，based on the highly conserved amino acid sequences FTQQYQ and anti-1/MIWPNV and the polymorphic sequences of S-alleles. To verify the accuracy of identified S-genotypes，reciprocal crosses-pollination experiments were carried out using‘Huarui’and ‘Huashuo’with‘Meiba’，‘Jinxiuhong’and‘Huaguan’，respectively. PCR results showed that when using the universal primers，two specific S-allele bands were amplified only from‘Fuji’，‘Huarui’，‘Huashuo’，‘Huaxing’，‘Meiba’and‘Hongcuibao’，whose S-genotypes were S1S9，S9S24，S5S9 and S5S24. When 19 pairs of specific primers were used for amplification，two specific bands were obtained from 14 cultivars，whose S-genotypes were S10S19，S2S3，S2S5，S3S10，S2S9，S5S24，S9S10，S3S10 and S5S9. Therefore，the S-genotypes for 20 apple cultivars were：S1S9 for‘Fuji’，S9S24 for‘Huarui’and ‘Huashuo’S5S9 for‘Huaxing’，‘Meiba’and‘Hongzhenzhu’，S5S24 for‘Hongcuibao’，‘Huayu’and‘99-1-29’，S10S19 for ‘Huashuai’，S2S3 for‘Jinyu’，S2S5 for‘Zaohong’，‘Huamei’and‘Gala’，S3S10 for‘Seokwang’，S2S9 for ‘Jinxiuhong’，‘Miyu’and‘Huaguan’，S9S10 for ‘Lüjia’，S3S10 for‘Shinano Red’，respectively. The pollination experiment showed that the fruiting rate of reciprocal crosses-pollination between‘Huarui’and ‘Huashuo’ germplasm was lower than 15.52% in 2015 and 2016. The fruit ratio of reciprocal crosses-pollination between‘Huarui’and‘Huashuo’with‘Meiba’，‘Jinxiuhong’，‘Huaguan’，‘Fuji’were higher than 46.30% in 2015 and 2016. Therefore，in this experiment，the same combination of S-genotypes had a low fruit setting percentage but a higher fruit setting percentage with different S-genotypes，indicating that the results of pollination supported the results of S-genotype identification. |
| |
| Keywords: | Malus × domestica self-incompatibility S-genotype |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
